Detection For Snps Of Canine Hip Dysplasia (CHD) And Nerve Pattern Related Genes In Labrador Retrievers

Objective: 1.To provide an efficient genetic diagnostics method for Labrador Retrievers,6 SNPs of 5 canine hip dysplasia(CHD)related genes in genomic DNA of Labrador Retrievers were detected.2.Detection of 7 SNPs associated to nerve pattern related 5 genes in Labrador Retrievers to provide an early-stage molecular genetic index for nerve pattern diagnostics.Methods: 1?11 normal and 6 typical CHD Labrador Retrievers were selected by imaging diagnosis.The vein blood was sampled and the genomic DNA was isolated from white blood cells.The PCR/sequencing method was used to analyze the variants of 6 SNPs in 5 canine hip dysplasia(CHD)related genes(LAMA2(CFA1:70997779),LRR1(CFA8:29247021),SCR(CFA24:28944481),COL6A3(CFA25:51031100,51040259),and FN1(CFA37:25095511)).Furthermore,analysis and calculate of the percentage of parting,differences in normal dogs and hip dysplasia.2?According to the behavior evaluation standards of China guide dog training center,the dogs belong to typical nerve patterns(quiet,active,and timid(weak)type,6 dogs each)were selected.The vein blood was sampled and the genomic DNA was isolated from blood cells.The PCR/sequencing and PCR-RFLP methods were used to analyze the variants of 7 SNPs in nerve pattern related 5 genes:(COMT(G482A),MAOB(T199C),SLC1A2(T471C)?ABCB1(A985T,T3442 C,377-378 ins C),GNB1L(961-962 ins G)).Furthermore,the association between the SNP variants and nerve patterns was analyzed by Chi-square Test.Results: 1?The variants of LAMA2(CFA1:70997779),LRR1(CFA8:29247021),SCR(CFA24:28944481),COL6A3(CFA25:51031100),and FN1(CFA37:25095511)SNP loci were not significantly associated to CHD.A/A variants in COL6A3(CFA25:51040259)SNP locus was significantly associated to CHD(>30%).2?G/G variants in COMT(G482A)SNP locus in the proportion of quiet dogs was significantly higher than that in timid dogs(P=0.026),but in the proportion of the two nerve patterns was separately no significantly different from active dogs(P > 0.05);G/A variants in COMT(G482A)SNP locus in the proportion of timid dogs was significantly higher than that in the active and quiet dogs(P=0.011,P=0.011);A/A variants in COMT(G482A)SNP locus in the proportion of active dogs was significantly higher than that in the quiet and timid dogs(P=0.008,P=0.008).MAOB(T199C)SNP locus in the proportion of quiet,active and timid dogs was no significant difference(P > 0.05).T/T variants in SLC1A2(T471C)SNP locus in the proportion of active dogs was significantly higher than that in the quiet and timid dogs(P=0.026,P=0.006);T/C variants in SLC1A2(T471C)SNP locus in the proportion of active dogs was significantly lower than that in the quiet and timid dogs(P=0.023,P=0.011);C/C variants in SLC1A2(T471C)SNP locus in the proportion of active dogs was significantly higher than that in the quiet and timid dogs(P=0.008,P=0.008).T/T variants in ABCB1(A985T)SNP locus in the proportion of active dogs was significantly higher than that in timid dogs(P=0.014),but in the proportion of the two nerve patterns was separately no significantly different from quiet dogs(P > 0.05);T/A variants in ABCB1(A985T)SNP locus in the proportion of active dogs was significantly lower than that in the quiet and timid dogs(P=0.005,P=0.002);A/A variants in ABCB1(A985T)SNP locus in the proportion of quiet,active and timid dogs was no significant difference(P > 0.05).T/T variants in ABCB1(T3442C)SNP locus in the proportion of active dogs was significantly higher than that in timid dogs(P=0.045),but in the proportion of the two nerve patterns was separately no significantly different from quiet dogs(P > 0.05);T/C variants in ABCB1(T3442C)SNP locus in the proportion of timid,quiet and active dogs was significantly different from each other,and in the proportion of timid dogs was highest(50%),and in the proportion of active dogs was lowest(0%);C/C variants in ABCB1(T3442C)SNP locus in the proportion ofquiet,active and timid dogs was no significant difference(P > 0.05).No ABCB1(377-378 ins C)and GNB1L(961-962 ins G)SNP loci was found.Conclusion: 1?A/A variants in COL6A3(CFA25:51040259)SNP locus might be used as a practical genetic detection index for CHD diagnostics in Labrador Retrievers.However,further confirmation in large-sample is required.2?G/A and A/A variants in COMT(G482A),SLC1A2(T471C),T/A variants in ABCB1(A985T)and T/C variants in ABCB1(T3442C)are significantly associated to nerve patterns and might be used as a molecular genetic index for nerve pattern diagnostics in Labrador Retrievers.However,further large sample analysis is required.