Community Diversity Of Endophyte Of Kiwifruit And Screening Of Antagonistic Strains

Agricultural plant protection information is information important part of the use of information technology, the development of modern agriculture has become an integral part of agricultural development. To promote the development of plant protection information, more precise, more specialized plant protection information gathering and research. In this paper, kiwi as research material, and its isolation and identification of endophytes, analyze the differences of cultivated and wild macaque endophytes and kiwi screening endophytic bacteria. The result is mainly used for prevention and treatment of diseases of kiwifruit plant protection information.In this study, during 2014 to 2015, in Hunan Province "Dongshanfeng" Jiangxi "Jinggangshan" wild kiwifruit and Sichuan Qionglai, Shifang, Dujiangyan kiwi garden varieties and other places, the health district collected kiwi and suspected diseased plants, was born in the kiwifruit bacteria isolated, purified and identified, constructed 16 SrDNA sequence amplification and analysis, the use of network information resources GenBank, analyzes the diversity of endophytes kiwi and kiwifruit canker bacteria were Oxford Cup law indoor screening indoor antagonistic experiments and branches of Actinidia control effect experiments, the results of this study are as follows:(1) sample from a different geographic location and altitude of a total collection of microorganisms isolated and purified within 413 Kiwi. The endophyte two wild kiwifruit growing region of reference features are divided into 24 groups were compared by analysis of sequencing results with GenBank database, the regional grouping Jinggangshan mainly Klebsiella sp., Sphingomonas sp., Pseudomonas sp., Agrobacterium sp., and other Xanthomonas campestris pv., Chryseobacterium sp. genus, strain Dongshanfeng region grouping mainly belong to Erwinia rhapontici, Novosphingobium sp., Pseudomonas sp., Xanthomonas campestris pv., Lysinibacillus sp., Bosea sp., Agrobacterium sp., Rahnella aquatilis, Frigoribacterium sp., Rhizobium sp., Microbacterium sp., xylophilus sp.,Caulobacter sp., Bacillus sp., etc. belong. By sequencing features and fully reflects the inner kiwi endophytes number of species richness.(2) from the separation of finishing than the number of bacteria in the distribution of raw kiwi difference. Obtain the following variation: the overall trend by number and type of distribution is the wild growth> orchards. Overall changes in the law according to their respective sampling partial quantity endophytes are presented: Buds> stems> leaves. Different types of sample kiwi endophytes of view, compared to the number of bacteria actinomycetes accounted for more benefits, 86.2 percent of the total. The whole area is the law of change: Dongshanfeng> Jinggangshan> Shifang> Dujiangyan> Pujiang; distribution is part of the buds> stem> leaf. Kiwi endophytic actinomycetes 13.8 per cent of the total number of endophytic bacteria, regional distribution rule is: Dongshanfeng> Jinggangshan> Dujiangyan> Pujiang> Shifang; distribution site is bud> leaves> stems. In addition, the kiwi endophytic bacteria will also be the number of sample separation is healthy kiwi strains or diseased plants influence, in general is healthy plants> endophytic bacteria number of diseased plants.(3) The results showed that the number of wild kiwifruit Jinggangshan its endophytes and richness in the highest range of 800-1000 meters above sea level, and is mainly concentrated in the eastern peaks below 800 m and 1000 m above; specific to the genus distribution, the two are isolated endophyte strains are Pseudomonas sp., Agrobacterium sp., Xanthomonas campestris pv. trigeneric the remaining species are not consistent. And all the strains of its two distribution altitude and has a large number of differences, which is consistent with the dominant populations Pseudomonas sp. Are the two regions.(4) by trial and error, screened antagonism good and stable six strains analyzed 16 S r DNA sequencing and GenBank, that number 62,113, 110 of Pseudomonas sp. and number 189,213,225 of Lysinibacillus sp.