Content Determination Method And Time Variation Of The Year Of Taxanes, Flavonoids And Polysaccharide In The Needles Of Taxus Chinensis Var.mairei

Taxus, locally named "yew", is an evergreen plant belonging to Taxaceae. Because of the high efficiency of paclitaxel, Taxus has attracted much attention. In addition to paclitaxel, the flavonoids and polysaccharides also have some antioxidant, anti-tumor and other activities. Research on taxanes, flavonoids and polysaccharides is helpful to improve the utilization of the resources of Taxus. In this study, the needles of Taxus chinensis var. mairei were used as material, content determination method and time variation of the year of taxanes, flavonoids and polysaccharide were studied. The main resultss are as follows:The satisfactory chromatographic result for taxanes was performed by using gradient elution with acetonitrile and water under a flow rate of 1.0 mL/min at 30?, detection wavelength at 227 nm on Zorbax Eclipse C18 Plus(4.6×250 mm,5 ?m). 10-DAB content was higher in January and November. The content of baccatin III in February and November is higher. 10-DAT content was higher in February and June. Cephalomannine content was highest in December. Paclitaxel content was higher in February and December. Overall, in order to obtain higher yield of taxanes, February, November or December is more suitable for harvest the needles of Taxus chinensis var. mairei.The satisfactory chromatographic result for quercetin, amentoflavone and ginkgetin was performed by using gradient elution with acetonitrile and water under a flow rate of 1.0 mL/min at 30?, detection wavelength at 360 nm on Zorbax Eclipse C18 Plus(4.6×250 mm,5 ?m). The content of ginkgetin was higher in August and November. Quercetin and amentoflavone content was low. The content of total flavonoids was higher in January, August and November. Overall, in order to obtain higher yield of flavonoids, August or November is more suitable for harvest the needles of Taxus chinensis var. mairei.A new method for the determination of Polysaccharides in Taxus chinensis var. mairei Leaves(PTL) has been devoloped. The optimum conditions were: detection wavelength at 460.0 nm, the concentration of NaOH, CPC were 1.0 mol/L and 1.0 mmol/L separately and incubation time was 10 min. The enhanced RLS intensity was proportional to the concentration of PTL in the range of 2.4~12 ?g/ml. The results showed that PTL content increased first then decline. In order to prepare high content polysaccharides, September to November is more suitable for harvest the needles of Taxus chinensis var. mairei.