| Pinus elliottii has good economic because of the higher and quality of the rosin and timber. In order to better understand the genetic background of 111 high fat American imported wetland pine families, this paper uses SSR markers from the level of DNA research the Pinus elliottii in genetic relationship which planted in the Jingdezhen Maple mountain forest and 111 high fat wetland in Ji'an Baiyun Mountain forest of pine sample.The study developed SSR primers of Pinus elliottii using Pinus EST database.Designed SSR primers and selected high polymorphsim primers.Using popgene software analysed the polymorphsim of SSR and SSR motifs.NTSYS clustered 111 families of the Pinus elliottii.The results are like that:(1)In this study,detected 6482 SSR containing sequences using the EST database, and the frequency is 5.37%. A total of 7542 SSR loci were detected, and the distribution frequency was 6.25%. The SSR repeat types were abundant in slash pine.The mononucleotide,dinucleotide and trinucleotide were the largest accounting for 97.24% of all SSR loci. Mononucleotide motifs accounted for 50.85%, dinucleotide motifs for 16.47%, trinucleotide motifs representing 29.93%, tetranucleotide, pentanucleotide and hexanucleotide with less content. Average SSR motif length distribute in 10-195 bp.The 10-13 bp accounted for the total number of 42.51% of Pinus elliottii, 14-17 bp motifs sequence accounted for 27.58%, 19-21 bp motifs sequence accounted for the total number of 14.26%, 22-195 bp accounted for the total number of 15.65%. There were 96 types of SSR motifs. The A / T accounted for 46.95%, G/C accounted for 3.9%, AT /AT total of 9.76% and dinucleotide accounted for 4.4%, AC/GT accounted for the total number of 2.16%, CG/CG accounted for the total number of 0.15%, AGC/CTG accounted for the total number of 6.36%, ACT/AGT accounted for the 0.46%.(2)This paper designed 256 pairs of SSR markers using Pinus plant EST database. Amplified 65 pairs of primers, efficiency 25.39%.8% polyacrylamide gel electrophoresis after rescreening screened a total of selected 39 polymorphic primers, and the polymorphism rate was 15.23%. As the latter part of the research of Pinus elliottii genetic relationship of EST-SSR molecular marker.(3) Selected 7 pairs of primers from 39 pairs of primers were clear and polymorphic in 111 large samples of Pinus elliottii. Using Popgenge software showed that the polymorphisms of 7 SSR primers and 100% were polymorphic. 7 SSR loci were detected in 111 of the 21 Pinus elliottii, and 3 alleles(NA) were detected on average. 7 SSR loci were amplified with 12.89 effective alleles(ne), among which the number of effective alleles was 2.74, while 4-3919 was 1.20. The average number of effective alleles per locus was 1.84. The Shannon diversity index(I) of the 1-5872 locus was the highest, which was 1.05, while the lowest 4-3919 was 0.332, and the average I value was 0.73. The expected heterozygosity(He) of the 1-5872 locus was highest for 0.64, while the 4-3919 was lowest at 0.17, with an average of 0.43 per locus for He. The observed heterozygosity(Ho) at 1-5872 loci was highest at 0.67, while the locus 4-3919 was lowest at 0.02, with an average of 0.40 per locus for Ho. Slash pine Nei 's diversity index(Nei' s) in the 0.349~ 0.6349, the average of 0.421-5872 of the Nei s' maximum of 0.63, the lowest for the 4-3919, only 0.17.(4)The study found that the relationship between No.5499 and No. 10699 sample and other sample is far more than that of other samples. 111 samples were classified into 7 categories the similarity coefficient is 0.62. No.1699 sample and No. 4398?1298 samples together as a class; No.1198, 3198 and No.6598, 7298, 10398, 10798 clustered into a class; No. 10599, 1999, 7199, 6299, 4799, 5899, 7699, 9799 samples and No. 10098 samples clustered into a class. No.11098, 8998, 6998, 4298, 2098 clustered into a class; No.54 99 samples together as a class; No. 10699 samples together as a class; the rest of the poly as a class. |
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