| Pollination is the necessary stage of seed plant fertilization, has yet to see the research on the Pollination Characteristics, pollination mechanism of Lespedeza davurica reports.The experiment with relatively large differences in a region, Shanxi Province, eight of wild Lespedeza davurica as the object of study on the pollination biology, from phenology, floral size, flowering dynamic, pollen viability, stigma receptivity, pollinators and other aspects studied Lespedeza davurica pollination characteristic, The main results are as follows:1. On growth a year and growth in population group of each phenological phase comparison can be found, the ranking group from seedling stage to branching stage of growth are basically the same, but starting from pregnant bud stage, differences in the growth on the show, two years growth to specific growth a year growth period length of 5?7 d. Among them, the population of Xiangfen population was the longest, which was 193 d, the shortest growth period in Zhongyang, only 171 d.2. As Lespedeza davurica flowers open with obvious asynchrony, generally from the base to the top in turn open, flowers open color is light yellow, larger flowers, with faint fragrance. A single flower with a shorter flowering stage, the petals are generally open during the day and at night. On the comparison of the size of the wing and the keel, it was found that: the 1 wings of the wings were 2 long, the keel was 1 and the keel was 2, and the petals showed obvious asymmetry. Stigma and anthers spatially separated. The number of flowering increased with the increase of temperature and humidity, and reached the highest peak at 14:00-12:00.3. By using the peroxidase precipitation assay, MTT assay and TTC and Aceto carmine method of pollen staining, showed that MTT staining method can be used for rapid determination of Lespedeza davurica pollen viability staining method. Effect of different storage conditions on Lespedeza davurica pollen viability was found in the?20? best, wet and dry conditions, dry storage treatment is better to dry. The pollen viability and stigma were observed and found that the anther was in the third stage. In stage V the viability and stigma receptivity is the best. Along with temperature and humidity decrease of Lespedeza davurica stigma can be pollinated and pollen viability will continue to rise, at 12:00?14:00 between the vitality of the highest value.4. Heshun ranking group of single pollen number up to 8750±1946 (grain/flower), and pollen quantities of Zhongyang least only 5370±879 (grain/flower). Population of different populations found that the growth of two years of the amount of pollen is generally more than one year of growth. Artificial pollination processing and display of Lespedeza davurica has a certain degree of selfing and outcrossing hybrid also has a certain degree of affinity, there is no apomixis phenomenon. According to the P/O value measured by Lespedeza davurica breeding system for obligate outcrossing. Hybrid index of Lespedeza davurica was 4, the breeding type for the existence of selfing and outcrossing, to spread pollen media.5. Insect visit behavior will appear in 10:00?11:00 between the first visit to the peak, in the afternoon 15:00?16:00 appear second times the peak, the first peak is less than second times. Hymenoptera insects such as bees and other pollinators most obvious effect. The effective transmission range of pollen is half a meter under the action of wind.The test of Lespedeza davurica pollination biology characteristics have a certain degree of understanding, in order to carry out the sexual hybridization, provides reference basis to formulate an effective breeding programme and a variety of excellent promotion and application. |
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