Damage Difference And Resistance Of Three Main Cultivated Wolfberry

The Aceria pallida Keifer is one of the key pests of the Lycium barbarum L.To understand correlation between mite damage and growth characteristics,physiological and biochemical changes of Lycium barbarum L.Three main cultivated wolfberry as experiment object,the gall area,the number of gall in per leaf and the number of the mite in per leaf were studied,and compare the difference of three main cultivated wolfberry.The growth characteristics,the leaf structure,the metabolites and enzymes about resistance were studied,and the difference was compared and analysed the relationship with the mite damage 1 The mite damage difference of three main cultivated wolfberryThe indoors and outdoors experiments indicated that there existed significant differences among three main cultivated wolfberry for mite damage,N ingqiNO.5> NingqiNO.7> NingqiNO.1.And there were significant differences among three main cultivated wolfberry for gall size,gall numbers per leaf and percentage of damage per leaf.When the mite damage was relative less,three main cultivated wolfberry showed mite damage differences in gall numbers per leaf and percentage of damage per leaf.But when the mite damage was serious,the percentage of damage per leaf was better than the gall numbers per leaf to show mite damage differences.113 galls were subjected to observation under LEIC A optical microscope and counted the mite numbers.The result showed that most galls have 30~430 mites,the least number of mites was 1 and the largest number of mites was 1274.The number of mites in mature galls was correlated with gall size.The number of mites increased with increasing the gall size.But three main cultivated wolfberry had no significant differences in the mite numbers per gall and the mite numbers per size.2 The growth characteristics of three main cultivated wolfberryThere were significant differences among three main cultivated wolfberry for germination,in which N ingqiNO.5 were earliest,NingqiNO.7 took second and NingqiNO.1 were latest.The germination of N ingqiNO.7 were 1~3 days later than the germination of NingqiNO.5.And the germination of N ingqiNO.1 were 3~5 days later than the germination of NingqiNO.5.There was a certain degree of correlation between the germination and the mite damage degree.Early germination of species are more susceptible to mite damage.There were significant differences among three main cultivated wolfberry for softwood.The shoot growth of three main cultivated wolfberry in the twelfth day was respectively 57.32 cm,127.93 cm,and 105.93 cm.There was a certain degree of correlatio n between the softwood and the mite damage degree.The more tender shoots of species are more susceptible to mite damage.But three main cultivated wolfberry have a similar growth dynamics.Their galls also had the process of forming,mature and decline.The results showed that in the initial period,the gall appeared yellow-green and the size increased quickly,but the number of mites in the galls increased slowly.The Mature galls were yellowish-brown,the gall size had no significant change.And the number of mites increased exponentially with the frequent phenomenon of mite migration 3 The leaf morphological structures of three main cultivated wolfberryThe Leaves of three main cultivated wolfberry were all strip lanceolate in shape.Epidermal cells have a relatively well-developed cuticle.There were no significant differences among three main cultivated wolfberry for stoma number.Three main cultivated wolfberry were similar in the microscopic structure of galls which is composed of epidermis,mesophyll and vein.The initial formation of galls: the epidermal cells of the infected surface were raised,to form a clear invasion of the channel.The palisade tissue and spongy tissue around mites were disappeared,forming a hollow chamber.The growing stage of galls: there was an obvious increase of insect galls with little mites.There were 2~3 layers arranged closely and similar circular parenchyma cells around the chamber.And the chloroplast was decreased in cells.The mature stage of galls: gall size was not increasing obviously,and the chamber was divided into small space through parenchyma cells.The vacuoles of the cells around the mites were absent,and the staining was deepened.After gall microstructure change was not obvious,but the mite increased rapidly.The decline stage of galls: galls appeared epidermal lignification,and there was mite frequent migration phenomenon.4 The contents difference of three main cultivated wolfberryThere were no significant differences among three main cultivated wolfberry for wax content.Compared with healthy plants,soluble sugar content of three main cultivated wolfberry were infested by mites were increased.There were significant differences among three main cultivated wolfberry,N ingqiNO.5> N ingqiNO.7> N ingqiNO.1.The content of soluble sugar and mite damage is closely related.Compared with healthy plants,total phenols content of three main cultivated wolfberry were infested by mites were increased.There were significant differences among three main cultivated wolfberry.The total phenolic content of NingqiNO.5 and N ingqiNO.7 increased respectively,and the total phenolic content of NingqiNO.1 increased very little.The total phenols content and mite damage is closely related.The total flavone content of N ingqiNO.5 and N ingqiNO.7 increased respectively,and the total flavone content of NingqiNO.1 decreased very little.The total flavone content and mite damage is closely related.Compared with healthy plants,there were significant differences among three main cultivated wolfberry for the POD increment.And there were no significant differences among three main cultivated wolfberry for the C AT increment.The CAT content and mite damage is not closely related.