Effects Of Exogenous Jasmonic Acid-induced Risistance On Aceria Palida Keifer And Natural Enemies

Aceria palida Keifer is a main pest of wolfberry which lives in a gall in its most livetime. It is very difficulty to estimate the population, so the relationship between gall size and the population is very important. Jasmonic acid is a kind of plant endogenous growth regulator. In recent years, numbers of studies have proved that the exogenous JA can induce plant resistance to insects, which is similar to that induced by herbivorous insects feeding. J A can induce plant direct and indirect resistance to insect pest. This study is to prove that the exogenous JA can induce wolfberry resistance, exploration of wolfberry in indoor and outdoor resistance response, while clearly effects of induced wolfberry risistance by exogenous JA on behaviors of Paratrioza sinica Yang et Li and Enderus sp..Galls from laboratory cultured wolfberry and field were measured and weighted. Dissect galls and count numbers of A. palida Keifer in it. Make statistical regression of relationship between numbers of A. palida Keifer and galls size, weight. Spray wolfberry cultured indoor with O.OOlmmol/L and0.1mmol/L JA, the contrast with water and acetone, and then place the wolfberry seedlings in wild and outdoor. Record the insect numbers, observing the species and quantity of the pests and beneficial insects landing on the seedlings. A. palida Keifer are put on the leaves of wolfberry respectively after spraying1d,5d,15d. Record the number, length and short size of the gall every three days and calculate the area and volume of galls. While use Y-type olfactometer to method for the behavioral responses of P. sinica Yang et Li and Enderus sp. of wolfberry with different JA induced.The results showed that the size of a galls increased gradually with time, but thickness changed a little. Most gall sized from0.1to3.0mm. Gall size had a significant relationship with A. palida Keifer numbers and the relationship of gall weight and the number of A. palida Keifer was most significant. The model of Y=15.116X5+9.441could be used to estimate the population of A. palida Keifer. The species and quantity of pest insects and natural enemies on the seedlings induced by exogenous JA have significant difference from the contrast and relate to the concentration of JA. The species of pests of induction group in wolfberry field were two kinds less than the contrast; compared with the contrast, the quantity of Lema decempuctata Gebler, P. sinica Yang et Li reduced significantly and the number of Phytoseiidae sp. increased significantly on induction for6days later. So it was the best that the resistance to insects was induced by0.1mmol/L JA for6days. And it was reducing12days later. The wolfberry induced by JA decreased occurrence of A. palida Keifer galls. With0.001mmol/L and0.1mmol/L JA, spraying5days the wolfberry induced resistance was most obvious, spraying15days the long diameter, short diameter, thickness and the area of the galls were inhibited at different degree. The growth of volume of galls on wolfberry seedlings sprayed by0.1mmol/L and0.001mmol/L JA was inhibited. In the behavior test, in addition to0.1mmol/L JA inducement on wolfberry at5th day make P. sinica away, other results of behavior responses tendency is not obvious.