Serology Survey And Isolation And Identification Of Goat Bluetongue Virus In Hubei

Objective:To determine the prevalence and provide a base for prevention in the goats of Hubei province based on serology and isolation of the pathogen.Method:Experiment of competitive enzyme-linked immunosorbent assay (c-ELISA) was adopted to investigate the seroprevalence of bluetongue virus in the goats of Yun Xi, Xing Shan, Yi Lin, Jin Zhou, Ma Cheng and Jiang Xia, seven regions of Hubei province. A total of 506 serum samples were collected. The antibody in vivo of bluetongue was tested by carrying out the Xing Shan was chosen as monitoring site of susceptible animal because the positive rate was close to the average positive rate. Virus was eventually isolated from BHK-21 cells after the proliferation by intravenous inoculation of chick embryos. Virus identification was done through nucleic acid test, restriction enzyme digestion, and sequence analysis.Results:A total of 130 positive serums of bluetongue antibody were detected by serological surveillance, with an average positive rate of 25.69%. In concerning of the geographic zones, the highest positive rate was 38.33% in Yun Xi, and the lowest was 4% in Jin Zhou. From April 2015 to November 2015, results from monitoring site showed that there was one sheep with positive bluetongue antibody. There has been an upward trend in positive rate during the test, and finally tended to stability. The highest rate of positive of antibody appeared in August. One strain virus was isolated from the blood samples before they had converted into positive. The isolated viruses was confirmed to be BTV through VP3 genes identified, named HB201508, with 94.9% highest similarity to DQ186828 and 80.2% lowest similarity to JN255914.Conclusion:The results showed that BTV have been widely existed in goats populations in Hubei, and different parts of the animals have different positive rates. One strain bluetongue virus was isolated from goats' blood samples that collected in the monitoring site, and provided an important evidence for further bluetongue prevention and control in Hubei province.