Serology Survey And Isolation And Identification Of Bluetongue Virus In Ruminants Of Guangxi Province

Bluetongue disease (BT) is a vector borne diseases which occur in ruminants without contact transmission. It caused by the bluetongue virus infection. BTV distribute widely in tropical and subtropical regions around the world. Although there were26serotypes in total, the major structural protein of the virus, VP7——the preferred antigen of BTV group specific serum antibodies——can induce in a group-specific antibodies.In this study, the antibodies of group-specific antigen VP7in serum was detected by agar gel immunodifusion (AGID) text,to understand the animal’s infection. The trial lasted three years, a total of6186sera include5602one from goat and584one from buffalo were collected. The sampling coverage in most areas of Guangxi. The result shows that although the distribution of seroprevalence of BTV in Guangxi ruminants is different from each other, the BTV infected ruminants distribute around area. The average seroprevalence rate was29.0%. Among them, the buffalo seropositivity rate is42.47%and the goat’s seropositivity rate is27.65%.Analysis of the serological survey results found that the seroprevalence of BTV in northern region is lower than in the southern region, and the mountain is lower than the plain and hilly; at the same time, the distribution of seropositivity rate relate to the climate factors such as the temperature and the rainfall. It shows that the prevalence of BT relate to the geography and climate.This study collected the ruminant’s blood samples inoculated with chick embryo and the sensitive cells. With this pathway, two virus——buffalo/LZ02/2011and goat/NN274/2011——are isolated successfully. Morphological identification, specific nucleic acid detection and immunoperoxidase staining methods confirmed that the two isolates are BTV strains, The virus titers were the of106.47TCID50/0.1ml and104.8TCID50/0.1ml.