Study And Evaluation On Innovation Cultivation Mode Technical Arrangement And The Quality Of Aucklandia Lappa Decne. In Si Chuan

Aucklandia lappa Decne. is from Compositae plant.The A. lappa Decne. of taste is acrid, bitter, warm,It has the efficacy of promoting the circulation of qi, relieving pain, invigorating the spleen, helping digesting.It has a long history of medicine, it is one of the main raw materials of Chinese patent medicine,such asIn recent years, because of its clinical application is more and more widely,market demand continues to expand,encouraged by the state to develop the policy of artificial cultivation of medicinal plant, in Xuanhan, Beichuan Lixian,Baoxing of Sichuan and in Kaixian,Nanchuan of Chongqing and other places have begun to widely cultivated.Because of the same kind of medicine in different natural environment, planting method, its intrinsic quality will have great difference,study comprehensive evaluation of the introduction cultivation of the quality of A. lappa Decne.in this topic,to clarify the quality of A. lappa Decne. after diffusion of producing area.to provide the basis for further expand the planting.The main contents of this paper are as following:1. Innovation cultivation mode technical arrangement of A. lappa Decne. of Sichuan based on"tail as seed"The traditional planting method of A.lappa Decne. is "seed cultivation method",Xuanhan of Sichuan recently summed up a set of "tail as seed" and "continuous cropping" methods,the cultivation technique was summarized in this paper,and declare patent,Finally,the key steps of planting technology in Xuanhan is summarized.2. Quality effect of the producing area diffusion of A. lappa Decne. by Grey Incidence Degree MethodAccording to the "Chinese Pharmacopoeia" (2015 edition) of the provisions about A. lappa Decne.,TLC identification of different cultivation patterns of Xuanhan and in different areas of A. lappa Decne.,and the moisture, ash, alcohol soluble and water soluble extract were examined, determination of costunolide, dehydrocostuslactonein and volatile oil content,and producing area in Lijiang and Xuanhan of heavy metal,trace elements and amino acids contents were determined. According to define the relative correlation degree, building quality evaluation model, combined with a number of indicators to evaluate the quality,and analysis of origin effect on the diffusion on quality of A. lappa Decne.The results show that the production of Xuanhan conforms to the "Chinese Pharmacopoeia" standard,the quality is close to Yunnan.3.Quality evaluation based on fingerprint of A. lappa Decne.of SichuanUsing high performance liquid chromatography (HPLC) method for 22 different regions of A. lappa Decne. materials make fingerprint analyses, and 22 of A. lappa Decne. common peak mode, analyses the test results, to establish a method to evaluate the quality of A. lappa Decne.of Sichuan based on fingerprint.4.Analysis of volatile oil and fat-soluble components of A. lappa Decne. of SichuanUsed GC-MS technology for the qualitative analysis research of the volatile components and fat-soluble components of 22 batches of A. lappa Decne.,90 kinds of volatile components were detected,mainly included terpenoids, alcohols and alkenes compounds.67 kinds of fat-soluble components were detected,mainly included fatty acid esters and hydrocarbons.5.Study on the extraction process of polysaccharide.Polysaccharide is a kind of compound which is widely existed in plants, previous studies have shown that it has a variety of effects on the human body,related health food development also gradually increased,the A. lappa Decne. have rich components of polysaccharide,the inulin has been used as micro index in the Chinese Pharmacopoeia to identify the authenticity of the A. lappa Decne., but long-term since polysaccharide as an active ingredient did not cause people's attention.In this paper, the A. lappa Decne.polysaccharide extraction process was optimized by central composite design and response surface method,to determine the best extraction process of polysaccharides,Xuanhan different cultivation patterns and different habitats of materials polysaccharides were extracted by the optimum conditions,and then determine the content of polysaccharide.To enrich the connotation of quality evaluation.