Influences Of Water Flows On Behavioral Characteristics,the Rate Of Oxygen Consumption And Blood Physiological Parameters Aspect Under Different Temperature Conditions Of Hemiculter Leucisculus

In the natural environment,water current is the main factor to promote the movement of fish since fish can adjust their swimming condition to adapt water environment when stimulated by current.To be specific,fish are able to regulate their swimming direction and speed based on the direction and speed of water flow so as to keep itself under the circumstance of counter-current swimming or staying in a certain position for a long while.This paper,accordingly,chose Hemiculter leucisculus as the subjects while the process of the velocity experiments was set up at the temperature of 12℃,18℃,24℃,30℃,totally four temperature gradients.Simultaneously,the impact of the current toward rate of oxygen consumption,tail beat frequency,physiological and biochemical indicators,at different temperature,were taken into research synthetically.Main results were summarized below:1.Effect of water flow to tail beat frequency for Hemiculter leucisculus.The frequency appeared to gently ascend when the rate raised from 0.5BL/s to 1.5BL/s and Hemiculter leucisculus turned to fit stably in this case of rate.Besides,when the current ranged from 1.5BL/s to 2.5BL/s,the trend of change increased as the frequency,together with the rate,grew distinctly and at this while of rapid rise,the fish had to accelerate their tail beat frequency in order to adapt the relatively strong flow.Nevertheless,the frequency descended slowly since the rate of stream exceeded 3BL/s.Temperature was positively correlated to tail beat frequency between 12℃ to 30℃ and the relationship went as F=1.672T-14.953,R2=0.860.2.Effect of water flow to oxygen consumption rate for Hemiulter leucisculus.The connection between the stream flow and oxygen consumption rate was determined through the automated oxygen consumption rate measuring of Swimming Tunnel(5L)respiratory system by AutoResp software.As the result displayed,a clear upward trend of the oxygen consumption rate appeared while Hemiculter leucisculus stimulated by the current.Oxygen consumption inclined to gradually go up when the rate rose from 0.5BL/s to 1.5BL/s and the metabolism of Hemiculter leucisculus was kept steady.Besides,when the current ranged from 1.5BL/s to 2.5BL/s,the trend of change increased as the oxygen consumption rate,together with the rate of stream,grew evidently and in this condition of rapid rise,the fish constantly expended their interior energy to overcome the obstacle of torrent.However,the rate of oxygen expense tended to decline if the rate of current was kept adding,and as a result of fact,this circumstance of physiological fatigue finally led to the fall of consumption.Temperature was positively correlated to oxygen consumption between 12℃to 30℃ and the relationship went as M02=8.671T-43.327,R2=0.975.3.Effect of water flow to blood physiological parameters for Hemiculter leucisculus.Variation was consistent for RBC,WBC,HGB and HCT within the blood of Hemiculter leucisculus as they all increased as the augment of flow rate,and reached maximum in 2.5BL/s.Yet the RBC,WBC,HGB and HCT of blood all revealed a continuous downward trend when the torrent continued to accelerate.Furthermore,no significant connection between the environment temperature and the physiological index of Hemmiculter leucisculus was reflected.Primary inference could be determined that,for Hemiculter leucisculus,the suitable rate of stream was 0.5BL/s-1.5BL/s and the suitable temperature was 12℃-30℃ through the research of the change of the water flow to tail beat frequency,oxygen consumption and physiological and biochemical indicators within blood.The level of metabolism and energy demand of Hemiculter leucisculus was perceived as the impact of water flow was taken into discussion.Simultaneously,aquaculture production,the improvements of environmental condition,long-distance transport and so on were thus equipped with theoretical support.Besides,relevant methods together with technical reference could be provided for the study of other fish in order to make contributions to the research and development of flow rate and fish metabolic theory.