Effect Of Monlchamus Alternatus(Hope) Harm On Pinus Thunbergii And Part Of Secondary Metabolites

Monochamus alternatus Hope is the important trunk borer of trees,and it's also the significant media of Bursaphelenchus xylophilus.It is primarily in the larval stage harm pine tree trunks,decay in adult eclosion period eating pine young branches,at the same time,in the process of spawning and feeding transmission were Bursasphelenchus xylophilus.Monochamus alternatus Hope will be induced in the process of feeding pine pine trees produce some defensive substances,which have flight from effects on pine sawyer brown.This article through studies the pine sawyer brown to weihai into forest farm Pinus thunbergii selective feeding place,at the same time the determination of the Monochamus alternatus Hope feeding hazard inducing pine produce changes of the defensive material composition and content and some of the defense material influence on Monochamus alternatus Hope in enzyme activity.The results are as follows:1.The study of the Monochamus adults' host selection from different parts of the pine behavior during feeding preferenceMonochamus alternatus Hope in large plants,south,east,west,north four directions of feeding area are: 21.8 cm2,21.5 cm2,15.3 cm2,9.8 cm2,east > south > west > north.Pine branches in the former of which is the size of parts was as follows: 14.9 cm2,33.5 cm2 and 18.9 cm2,namely,central branch > branches > branch at the front in the back.Pine sawyer brown on the pine tree,middle and bottom feeding area of 19.8 cm2,respectively,26.3 cm2 and 19.7 cm2,namely the middle > upper > lower.2.Monochamus alternatus Hope after feeding on the pine needles tannins,flavonoids,total phenol contentPinus thunbergii needles with pine trees suffer degree aggravating,the tannin content in changing.Needles in moderate tannin content when suffer the highest 0.549?g/ml,tannin content in severe harm minimum 0.326?g /ml,but those in mild tannin content 0.4?g /ml than healthy pine needle high tannin content 0.386?g/ml.Pinus thunbergii needles in the content of flavonoids as the victim degree aggravating constantly changing,mild suffer when flavonoid content increased,but decreased to 0.936?g/ml,in moderate suffer when the content of severe harm in flavonoids content is 0.996?g /ml.Needles in mild suffer flavonoids content is the highest,1.568?g/ml;In moderate harm flavonoids content is the lowest,0.936?g/ml.Pinus thunbergii needles of total phenol content as the victim degree aggravating content changes,different degree of victims Pinus thunbergii needles were increased,the total phenol content in needles in mild suffer the highest total phenol content,0.734?g/ml;Severe harm to the total phenol content is lower than the mild to moderate the hazards of content.3.The effects of Monochamus alternatus Hope feeding on pine secondary volatile substancesMonochamus alternatus Hope after feeding hazards,mechanical damage and health pine needles,in dealing with 36 h,12 h,24 h,48 h after volatiles type changing,a total of ninety-five kinds of detected.Among them,the most is terpene class material,at the same time also detected five alcohols,esters,five kinds of acid class one,ethers two kinds.The change of terpene class material is more complex,most of the terpenoid substances within a relatively short time after bite to eat all have varying degrees of increase or decrease.4.The Monochamus alternatus Hope 's choice of five secondary single terpenoids tropismRespectively for five different standard of compound monomer odor source control one-way choice experiment was carried out with air,Monochamus alternatus Hope adult to beta pinene,basil ene,selection of beta myrcene choice respectively 30 times more than air,26 times,27times,of limonene,beta-water dropwort the choice of material is less than the choice of air at 17 or 18 times respectively.By the difference analysis of variance,the choice of beta pinene strongest tropism,its selective extremely significant difference,the selectivity of basil and beta myrcene is higher also,choose the difference is obvious.Secondary single terpenoids which suggests that different effect on the Monochamus alternatus Hope adult seduce is different,the beta pinene,basil ene,beta myrcene lure of Monochamus alternatus Hope adult have obvious effect,and limonene,beta-water dropwort ene of Monochamus alternatus Hope adult no temptation.5.The effects of three kinds of secondary substances in Monochamus alternatus Hope larvae in brown detoxification enzymes and target enzyme activityMonochamus alternatus Hope larvae feeding containing 0.01% Rutin,0.01% flavonols and 0.01%total phenolics flavonoids rutin gallic acid artificial feed after 24 h,pine sawyer brown larvae of detoxifying enzyme activity of carboxylesterase,respectively,compared with the control group by 0.30,0.29,0.003 U/mg protein.Pine sawyer brown larvae target enzyme acetyl cholinesterase activity higher than the control group were respectively 0.08,0.008,0.07 U/mg protein.