Mass-rearing Of Dastarcus Helophoroides With Substitute Hosts

Dastarcus helophoroides(Fairmaire)belonged to coleoptera and bothrideridae are effective natural enemies to prevent and control a variety of forest borers.In this study,we chose three kinds of alternative host-Tenebrio molitor(pupae),Zophobas atratus(pupae),Arhopalus rusticus(larvae,pupae)for indoor expansion of dastarcus helophoroides.The amount of inoculated insects and the cocoon rate,emergence rates,adult male to female ratio,pre-spawning,single female fecundity,best picking ratio of breeding dastarcus helophoroides in different vaccination ratio were executed statistical analysis.The results are shown as follows:1? Single-headed Zophobas atratus pupa could breed up to 23 dastarcus helophoroides adult mostly,so it had the greatest potential to breed dastarcus helophoroides as an alternative host.Single-headed tenebrio molitor pupae could breed up to 5 dastarcus helophoroides adult,single-headed Arhopalus rusticus larvae could breed up to 5 dastarcus helophoroides adult,single-headed Arhopalus rusticus pupae could breed up to 9 dastarcus helophoroides adult.2?The cocoon rate and emergence rates were the highest when the inoculation rate of larvae at initial incubation of Zophobas atratus pupa and dastarcus helophoroides was 1:5,the rate were 72.22% and 69.11%,respectively.The highest feather rate was 35% when the inoculation rate of larvae at initial incubation of tenebrio molitor pupa and dastarcus helophoroides was 1:2,the highest cocoon rate was 39.33% and the highest emergence rates was 21.56% when the inoculation ratio was 1:5,the inoculation ratio was best at 1:2.The highest emergence rates was 74.44% when the inoculation rate of larvae at initial incubation of Arhopalus rusticus pupae and dastarcus helophoroides was 1:4.The cocoon rate continues to rise as the increase in the number of inoculation,up to 79.44%.The cocoon rate and emergence rates of dastarcus helophoroides bred by Arhopalus rusticus larvae were 69.72%and 61.11% respectively when the inoculation ratio was 1: 4 at the age of 9 larvae.3? The average body weight of dastarcus helophoroides adult bred by three kinds of alternative hosts was gradually decreased with the increase of the number of feathering.4 ? There was no significant difference in the length of spawning of dastarcus helophoroides bred by three kinds of alternative hosts,both about 40 days.The odds ratio of male and female of dastarcus helophoroides was not significant,which were close to 1:1.5? The difference of mortality was not significant from September to March of next year when different kinds of dastarcus helophoroides adults were fed with three kinds of artificial diet SL1,SL2 and SL3.There were no significant differences in the rates of monthly average single female fecundity through feeding different kinds of artificial diets.Three kinds of artificial diets could be used for the feeding of dastarcus helophoroides adults.6?At a period of seven months from September to March of next year,the spawning reached to the peak in September and the largest number of spawning more than 400 evenly when different kinds of dastarcus helophoroides adults were fed with identical artificial diet SL3,but the number of spawning was lowest from November to December,and parts of dastarcus helophoroides females had no spawning phenomenon,the fecundity gradually picked up after January.7? The emergence rates gradually decreased of inoculated dastarcus helophoroides as the increase of duration after pupation of host when inoculation ratio was same.The speed of epidermal sclerosis of tenebrio molitor pupae was the fastest.The optimal inoculation time of three kinds of alternative host pupae was 24 h after pupation.