The Genetic Diversity Of ALK Gene Control Rice Gelatinization Temperature And Its Effects On The Physicochemical Quality

It has very important significance to research the genetic diversity of rice resources and to analy genetic information for the rice breeding research and its resources protection.Gelatinization temperature was one of important indexes of rice cooking quality which was mainly controlled by ALK.In the paper,546 rice resources as the research materials were selected from all over the world included Yunnan Hani terraced fields,which were ones of wild and cultivated.The genetic diversity of ALK and the relationship between gene mutation types were analyzed on the physic-chemical quality.The mainly results were as followed:The result of the physic-chemical quality analysis indicated:all the eigenvalue were widely distributed,and also the quality distribution of international rice wider and richer than Yunnan rice,which was a simple background distribution,and the gelatinization temperature appeared big difference,i.e very low or very high value under the combination effect of gene and environment as compared to the Yunnan rice.In different subgroups,the gelatinization temperature of AUS rice and AROMATIC rice was higher,and the indica rice was higher than japonica rice;The content of apparent amylose was a widely distribution in rices of Indica,Japonica and ADMIX groups,and the AUS rice group involved to the secondary and higher content,and the AROMATIC rice group was mainly in moderate levels.The data of the RVA showed that the viscosity of Indica rice groups was higher than Japonica in the low temperature.The current cultivation rice of Yunnan Hani terraced fields could be divided into Japonica group(group ?)and Indica group(group ?)which accounting for 83%.The result also showed that the most of the varieties from Indica group contained the G-GC allele tend to be high gelatinization temperature compared with the varieties from Japonica group.The gelatinization temperature in selected cultivated rice materials was mainly related to three SNPs in the exon 8(4211G/A,4342G/T and 4343 C/T)in ALK,the combination of A-GC or G-TT types controled the very low and lower gelatinization temperature,while the combination of G-GC controlled very high and higher gelatinization temperature.As the traditional cultivated rice,the genetic polymorphism of Yunnan rice was lower.Wild rice was not domesticated and choiced,so its ALK haploid polymorphism was more richer than international and Yunnan rice.Its DNA polymorphism was higher,haploid type was rich,and three type alleles overall analysis of the ALK.Three alleles distribution varied between different subgroups in international rice.Preliminary concluded A-GC and A-TT type were split from G-GC type in the course of evolution,which the evolution was from Indica to Japonica.The 12 combination types of ALK and Wx and the quality respectively were explained in the paper,and the ALK and Wx maybe could have a certain chain relationship.