Relationship Analysis Of The Udder Bacterial Community Of Dairy Cows With Its Feed,Soil And Fecal Bacterial Community

In order to analyze structural characteristics and possible influencing factors of breast bacterial flora of dairy cows,a total of 9 Holstein cows were randomly selected from a large ranch in Hohhot to collect milk,feces,feed and soil samples.The structure of samples was analyzed by high-throughput sequencing.The results showed that 156 bacterial families were checked out in four kinds of samples on level of family.Among them,76 bacterial families were detected from milk samples,and dominant families were Moraxellaceae,Flavobacteriaceae,Rhizobiaceae and Caulobacter,accounting for 49%,15%,14%and 9%,respectively.25 bacterial families were detected from stool samples,and dominant families were Ruminococcus,Rikenellaceae and Lachnospiraceae,accounting for 46%,11%and 7%,respectively.84 bacterial families were detected from feed samples,and the dominant families were Enter obacteriaceae,Sphingomonadaceae,Rhizobiaceae,Pseudomonadaceae,Rhodobacteraceae and Lactobacillaceae,accounting for 16%,9%,8%,7%.6%and 6%respectively.89 bacterial families were detected from soil samples,and dominant strains were Nocardioides,Encapsulated bacteria,Micrococcaceae.Planococcus and Sphingomonadaceae,accounting for 9%,7%,6%,6%and 5%respectively.Comparison between samples showed that there were 15 common bacterial families in milk,feces,feed and soil samples,accounting for(similarity)11%,and dominant family was Mora.There were 17 common bacterial families in milk samples and feces samples,accounting for 34%,and dominant bacterial family was Moraxellaceae;There were 67 common bacterial families in milk samples and feed samples,accounting for 84%,and dominant bacterial family was Moraxellaceae;There were 68 common bacterial families in milk samples and soil samples,accounting for 82%,and dominant bacterial family was Moraxellaceae.The results showed that the similarity between milk and feed samples was the highest.The composition of flora of breast was closely related to the flora of feed,indicating that composition of flora in feed may influence composition of flora in breast.It provides a theoretical basis for the prevention of mastitis in dairy cows.