| As a traditional ornamental and medical plant,Paeonia lactiflora has been popular for long history,especially for cutting peony flower,which has good market prospect.We selected 65 peony cultivars and their wild species for morphological observation to make the classification key.Moreover,we reselected 22 cultivars for breeding materials for cutting peony to study the relationship between the cultivars and the wild species.The results of the study were as follows:Firstly: the leaf edge spine is as the species character for identification of P.lactilfora.Secondarly: The cultivars whose carpel was glabrous or pilose were in the subgroup of P.lactiflora or the subgroup of P.lactiflora.var.trichocarpa.In conclusion,26 cultivars were in the subgroup of P.lactiflora,36 cultivars were in the subgroup of P.lactiflora.var.trichocarpa,and 3 cultivars were in the subgroup of cultivars with carpel petaloid.Thirdly: based on the morphological characteristics of leaf margin and carpel as the main traits of cultivar classification,combining with flower shape and color,we set the classification key of herbaceous peony cultivars,aiming to provide a basis for cultivar identification,application and breeding.Fourthly: two main candidate DNA barcodes(ITS2 and psbA-trnH)were tested to identify twenty-one cutting cultivars of P.lactiflora and their wild species.ITS2 performed well in the PCR amplification,sequencing and the identification of cultivars from the Blast and phylogenetic analysis.Fifthly: The relationship of most cultivars in morphological study was similar to the the result of molecular marker method,which also showed close relationship with P.lactiflora,but partial cultivars showed different relationship between the two methods,which suggested the limitation of single classification method.Morover,it also reflected the complex relationship between the P.lactilflora and P.lactiflora.var.trichocarpa cultivars,implied the cross or transition among their parentage lineage. |
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