Establishment Of Paeonia Lactiflora Pall. Rejuvenation System

Paeonia lactiflora is a perennial perennial herb,which is belonging to Whole leaf subgroup,P.lactiflora group,P.lactiflora it is a collection of ornamental value,medicinal value and economic value in one,it is also an indispensable herbaceous flower in landscaping.P.lactiflora differs from other herbaceous plants in its complex dormancy characteristics,therefore,the proliferation and growth of P.lactiflora were restricted.For a long time,the breeding process and industrial development of P.lactiflora have been restricted because of its low reproduction coefficient and long breeding cycle,therefore,a complete and efficient regeneration system has not been established.In this study,the hybrid seeds of Fenyunu were used as the female parent and the female was the male parent,the regeneration system of P.lactiflora was constructed and discussed through three different regeneration ways.The main results of the experiment are as follows:1.The most suitable embryo starting medium for P.lactiflora is MS+6-BA 1.0mg/L+GA₃ 0.5mg/L,the germination rate of P.lactiflora was more than 90%,which increased the germination rate of P.lactiflora and broke the dormancy of P.lactiflora seed,The germinating rate of 40%was obviously superior to that under natural conditions;The optimum culture medium for embryo plantlet formation was determined by screening the kinin KT is MS+KT 1.0mg/L+GA₃ 0.5mg/L,the induction rate was 49.82%.2.The optimal medium for callus induction from cotyledon nodes of P.lactiflora is:1/2MS+NAA0.5mg/L+TDZ 0.2mg/L,The highest induction rate was 81.52%;The optimum medium for callus proliferation of cotyledons is MS+2,4-D 0.5mg/L+KT 1.0mg/L,Its multiplication coefficient was the highest,the highest was 2.08,and the browning rate was 21.73.3.On the culture medium of MS+6-BA 1.0mg/L+GA₃ 0.5mg/L,embryo inoculation of P.lactiflora,the germinating speed was the fastest and the effect of inducing cluster buds was the best.60 days later,transfer it to Z2 medium of MS+6-BA 2.0mg/L+GA₃ 0.5mg/L+NAA 0.2mg/L,so as to carry out proliferation and culture of cluster buds.The most suitable induction medium for cotyledons was found MS+6-BA 1.0mg/L+GA₃ 0.5mg/L+TDZ 0.5mg/L,Optimum rooting medium for tufted buds is1/2MS+6-BA 2.0mg/L+IBA 1.0mg/L+PVP 1.0g/L,it is beneficial to reduce the browning degree of explants with the addition of PVP 1.0g/L.4.Phytohormone,such as concentration of NAA,6-BA,KT,and CH,the effect on the embryogenic callus transformation rate of P.lactiflora reached a significant level?P<0.05?,The degree of influence of each factor is as follows:CH?KT?6-BA?NAA,the optimum medium for embryogenic callus transformation rate was found to be the best medium for embryogenic callus transformation rates:MS+NAA 0.1mg/L+KT 2.0mg/L+6-BA 0.5mg/L.