Investigation And Pathological Diagnosis Of Sheep Pulmonary Adenomatosis And Maedi-visna Disease In Part Areas Of Xinjiang

Sheep lung adenoma disease is a kind of infectious disease mainly caused sheep lung disease.it is widely existed in the world and is popular in Xinjiang and other places in China.The sheep meadi-visna disease mainly causes the chronic,progressive,infectious multiple organ damage syndrome,and its latent period is long and persistent infection in sheep.Because both of the two diseases cause chronic and progressive pneumonia symptoms and its clinical symptoms are very similar,it is hard to effectively identify according to clinical observation.In 2013-2015,The meadi-visna disease in five sheep breeds(Suffolk sheep,Dorset sheep,Kazak sheep,Hu sheep and Altay sheep)in Xinjiang were tested by ELISA and PCR.the positive rate of ELISA in testing Meadi-visna disease was 5.36 %(26 / 485).the positive rate of PCR in testing Meadi-visna virus was 3.71 %(18 / 485).the total infection rate of Mvv by PCR was low in different sheep breeds and infection rate of Suffolk sheep was 11.48 %(7 / 61)higher than other sheep breeds.In 2013-2014,The sheep pulmonary adenoma in six sheep breeds(Suffolk sheep,Dorset sheep,Kazak sheep,Hu sheep,Merino sheep,Emil white sheep)in six regions were tested by PCR,the total positive infection rate was 78.18 %(1981 / 2,534),DNA sequencing analysis shows that it belong to endogenous sheep pulmonary adenoma virus.The infection rate of Merino and Hu sheep was higher than other sheep breeds.11 suspected cases of sheep pulmonary adenomatosis and Maedi-Visna Disease were PCR detecting and histopathological observations,4 cases were diagnosed as sheep pulmonary adenomatosis,3 cases were diagnosed as Maedi-Visna Disease.Sick sheep nose flowed out a large number of liquid with thin foam,the volume of lung enlarged 2-3 times,gray or white nodular lesions scattered in part of lung.Alveolar epithelium and terminal bronchial epithelium hyperplasia and the formation of multiple papillary cystadenoma structure,adenoma around the alveoli filled with a large number of macrophages in sheep lung adenomatosis.Sick sheep nasal fluid flowed less,mostly dry cough,the lung volume increased,small tiny nodules scattered in part of lung.Microscopic observation shows lymphatic follicles enlargement and lymphoid reticular cell proliferation in Maedi-Visna Disease.The investigation of sheep pulmonary adenomatosis and Meadi-visna disease was carried out through molecular epidemiology and clinical pathology methods,The results showed that in some areas of Xinjiang,pulmonary adenomas mainly were non-infective,and the prevalence of Mady-Vesna disease was higher in sheep breeds imported locally.The detection of Mady-Visner's disease and pulmonary adenoma can be effectively diagnosed by PCR combined with histopathological observation,providing technical support for the differential diagnosis of these two diseases.