Control Technology Of Three Nuisanceless Insecticides On Anoplophora Glabripennis

Anoplophora glabripennis(Asian long-horned beetle,ALB)is a kind of wood boring pest,they are also an extremely invasive and harmful species,native to China and Korea,they have invaded America and some European countries at present.Once ALB invade to a new environment and colonize successfully,large areas of forest would be destroyed by them and they would cause enormous economic losses without artificial control.In the period of the occurrence of Cerambycidae pest,prevention measures is mainly for chemical control,with the growing awareness of people to protect the environment,at present,it has restricted the use of high residue and toxic insecticides.In order to explore new nuisanceless insecticides on ALB,three high efficiency,low toxicity,environmentally-friendly insecticides were selected in this test,they are chlorantraniliprole,flubendiamide and imidacloprid.The research contents in this thesis are control effects of three insecticides on ALB larvae and adults and effects of three insecticides on the activities of two detoxifying enzymes in ALB larvae.The main results are as follows:1.Field control efficiencies of three systemic insecticides via trunk infusion against ALB.Salix matsudana is one of the damaged tree species and it was selected in this test.A series of toxicity test with following three systemic insecticides by trunk infusion at three mass concentrations each: chlorantraniliprole(2,4,8 g/L),flubendiamide(0.6,1.2,2.4 g/L)and imidacloprid(2.8,5.6,11.2 g/L)were carried out against A.glabripennis larvae in the field,the control group was treated with 10% ethanol solution.The overall control efficiencies of chlorantraniliprole,flubendiamide and imidacloprid on the larvae were 37.7%~54.5%,41.9%~66.0% and 59.1%~86.4%,respectively.After prevetion,there was no new oviposition pit on the trunks treated with chlorantraniliprole or flubendiamide,there were new oviposition pits on one-seventh of the total trunks treated with imidacloprid,there were new oviposition pits on 50%~60% of the total trunks in the control group,the oviposition pits densities were 2~2.2 pits/tree.The result showed that these three systematic had repellent effect on oviposition behavior of adults in different levels.Therefore,these systemic insecticides are potentially useful for effectively controlling ALB population densities and reducing their damages to forests.2.Effects of three insecticides on the activities of two detoxifying enzymes in ALB larvae.The trees were treated with three insecticides,the control group was treated with 10% ethanol solution,after 16 days the larvae in the treated trees were collected,the carboxylesterase(Car E)and glutathione s-transferase(GSTs)activities in larvae were determined.The following result were:(1)Car E activities of larvae in the chlorantraniliprole and flubendiamide groups were all significantly higher than that in the control group,activity in the imidacloprid group was lower than that in the control group but not significant;(2)GSTs activity in the flubendiamide group was significantly lower than that in the control group,activity in the chlorantraniliprole group was lower than that in the control group but not significant,activity in the imidacloprid group was higher than that in the control group but not significant.These results showed that,chlorantraniliprole and flubendiamide could promote Car E activity and could inhibit GSTs activity in A.glabripennis larvae;imidacloprid could inhibit Car E activity and could promote GSTs activity in ALB larvae.3.Laboratory evaluation of the toxicity of three insecticides for control ALB.A series of laboratory bioassays were conducted on following three insecticides,each was sprayed onto the Salix twigs at three different dilutions: imidacloprid(20000,30000,50000),chlorantraniliprole(100,300,500)and flubendiamide(2500,3750,5000),the control group was sprayed with tap water.Our results showed that the overall average longevities of the ALB adults was 1.39~2.89 d in the imidacloprid group,1.72~2.97 d in the chlorantraniliprole group and 1.72~3.78 d in the flubendiamide group,respectively,which was significantly shorter than that in the control group(10.18 d).The ALB adult mortalities in the treatment groups reached 100% within 6 days.The twig consumptions by ALB adults in the treatment groups were either zeros or significantly lower than that in the control group.These results showed that these three insecticides exhibited strongly toxicity against ALB adults,and might have a great potential for use the pest control program.