Study On The Environment Response Of Anemone Obtusiloba Flower Color In Alpine Meadow Plants

Anemone obtusiloba D.Don.is a perennial herb unique to Anemone L.of the genus Ranunculaceae of the Tibetan Plateau.It has a certain medicinal value and is white and light.Yellow and yellow flowers.The Tibetan Plateau,where the A.obtusiloba grows,has a bad environment,a cold and changeable climate,a simple and fragile ecological system,and has obvious climate change in recent years.As an herbaceous plant in the re gion,A.obtusiloba has undergone significant changes in its suitability to the environment: White flowers have been significantly reduced,and at higher elevations,Maqu has not even seen white flowers,and the color has been significantly deepened,reflecting its the response to the environment.In this paper,ultraviolet visible spectrophotometry,Ultra Performance Liquid Chromatography(UPLC-PAD)and liquid chromatography-mass spectrometry(LC-MS)were used to analyze the different color buds of A.obtusiloba at different altitudes in Hezuo and at Maqu.The anthocyanin composition explores the main pigments that affect the color change and its response to the environment.Obtain the following experimental results:1.Ultra high performance liquid chromatography was used to determine flavonoids.Five samples of A.obtusiloba were detected at a wavelength of 300 nm.Seven compounds were detected in yellow of Maqu,yellow,and light colors of Hezuo.The peak time of the seven compounds was basically the same;the absorption peaks of flavonoids and carotenoids in the yellow,light yellow of Maqu and yellow,pale yellow and white flowers of Hezuo were all decreased with the lightness of the flowers;the A.obtusiloba flavonoids in the two regions showed the maximum absorption peaks at 268 nm and 330 nm,the main peaks at 240-285nm(peak zone II)were more prominent,the weaker bands at 300-350nm(peak band I),and the A-cyclobenzoyl system.(peak band II)and B-ring cinnamoide system(peak band I)both contain A-cyclobenzoyl system with high intensity;The maximum absorption peak of carotenoids appeared at 268nm;The total content of flavonoids was significantly higher than that of carotenoids,and the changing trend was more obvious.2.Ultra high performance liquid chromatography was used to determine flavonoids.Five samples of Anisoptera wilfordii were detected at a wavelength of 300 nm.Seven compounds were detected in yellow of Maqu,yellow,and light colors of Hezuo.Eight compounds were detected in the Hezuo white and Maqu light colors.The peak time of the eight compounds was basically the same,but the absorbance value changed greatly,and the content of each component decreased with the lighter color.Peak 3 and peak 8 may be isomers.Different plots with different flowers of A.obtusiloba the flavonoids may have the same type of flavonoids.3.Maqu A.obtusiloba with flower color shallow compound content reduced obviously is compound represented by peak 1,peak 3,peak 4 and peak 5;Hezuo A.obtusiloba flower color with shallow compound content reduced obviously is compound represented by peak 1,peak 2,peak 3,peak 5 and peak 6.In different altitude,found in Maq u,Hezuo between the two A.obtusiloba color difference is mainly composed of compound peak 3,peak 4 and peak 7 representative compounds.4.LC-MS determination of 7 kinds of anthocyanidin: luteolin-3-7-O-diglucoside: molecular ion m/z 645.18([M+H]+)peak time of 31.88 min;quercetin-3-O-rutinoside: molecular ion m/z 625.15([M]+)peak time of 33.24 min;kaempferol-3-O-sophoroside: molecular ion m/z 611.2([M+H]+)peak time of 36.19 min;myricetin-3-O-rhamnose: molecular ion m/z 483.1([M+3H]+)peak time of 42.58 min;quercetin-3-O-galactosidase: molecular ion m/z([M+H] +)465.1 peak time of 45.31 min;quercetin-3-O-glucoside: molecular ion m/z 465.1([M+H]+)peak time of 45.74 min;quercetin-3-O-rhamnolipid: molecular ion m/z 449.2([M+H]+)peak time of 47.40 min.5.According to the results of ultra-high performance liquid chromatography and liquid chromatography and mass spectrometry,the quercetin has the largest proportion of sugar derivatives and the most obvious change with the change of flower color.It is the main chromogenic pigment that causes the difference in flower color of A.obtusiloba.According to the flavonoid metabolic pathways,there are three main ways to increase quercetin:(1)reduce the activity of Flavone Synthase II(FS?)or attenuate the translation of Flavone Synthase II gene to reduce the path of Eriodictyol;(2)Enhancing the affinity of Flavonol Synthase(FLS)and Dihydroquercetin makes Dihydroquercetin superior in substrate competition;(3)Enhancing the translation of flavonoid 3?-hydroxylase(F3' H)genes,such as increasing the transcription factor in the promoter region to increase the content or activity of flavonoid 3 ?-hydroxylase to increase dihydroquercetin and saugenin The content.