| Paeonia lactiflora Pall. is one of China’s most traditional flowers. Seeds which were collectedfrom peony cultivars ‘Zi feng Yu’,,‘Fen pan cang zhu’,‘Lu hong’,‘Da fu gui’ and wild species P.veitchii were used to break the embryo’s dormancy and continue to systemic research on thedevelopment of embryo. Peony cultivars ‘Fen fan cang zhu’ and ‘Qi hua lu shuang’,‘Dan feng’,‘Luhong’ were used to discusses the bulbil disinfection procedures, Browning inhibition, bulbilgermination and axillary bud proliferation, rooting culture. These things provide the basis for thefactory micropropagation of peony superior cultivars. Different explants were used to induct callusand screened callus multiplication culture medium.In this article, regeneration system was constructed through embryo culture and cultivation ofbulbil built, and obtained the callus from different explants. The regeneration system providetechnical basis for factory micropropagation production, the foundation for further geneticengineering and cultivation of new cultivars of peony.The main results were as follows:（1） The different explants disinfection process was determinedExplants disinfection difference is in different, mature embryos treated with10%sodiumhypochlorite solution for15min, main shoots treated with2%sodium hypochlorite solution for10min. No strains of embryo and the callus induction of leaf, root, etc, only need75%ethanolprocessing15s.（2） Investigated the types of embryos germination influence factors and selected optimalgermination of embryo culture mediumSeed coat is correlated with embryo germination, embryo withtout seed coat were easier togerminate. The germination rate of light conditions is higher than that the dark condition,illumination has the certain promoter action for Paeonia lactiflora embryo germination; Germinationrate of different embryo which were picked on different peony cultivars exist differences: theselected four cultivars kinds of embryo germination rate at the highest of ‘Da fu gui’,‘Bian di hong’and ‘Qi hua lu shuang’ are lower,‘Die luo fen chi’ is lowest.P. veitchii germination rate is lower than ‘Zi feng yu’, but the rite is better than ‘Zi Feng yu’.The best germination medium of ‘Zi feng yu’ is MS+GA31.0mg·L-1+6-BA2.0mg·L-1（NO.7）; Thebest germination medium of P. veitchii is MS+GA32.0mg·L-1+6-BA0.5mg·L-1（NO.9） The optimalrooting culture medium for germ is MS+GA30.2mg·L-1（NO.15）.（3） Established the bulbil micropropagation system ①The study of Browning issue in primary culturePVP and activated carbon is obvious good for Browning inhibition. Browning rate was as lowas5.00%, and activated carbon has the certain promoter action on bulbil germination; In addition,the explant material cutting with ascorbic acid solution, soaking in water for a long time andculturing in dark environment for a week, can effectively reduce the Browning rate.②The research of shoot proliferation systemObtained ideal germination conditions with ‘Fen yu nu’ bulbil; As main lateral buds and lateralbuds have different hormone response, so we screen their best culture medium separately. Lateralbud obtained ideal germination rate （100%）,‘Lu hong’ main buds in primary culture obtainedaxillary bud regeneration, the increment rate up to3.17.The best main buds germination medium of ‘Fen yu nu’ is1/2MS+GA31.0mg·L-1+6-BA1.5mg·L-1+NAA0.0mg·L-1+AC1.0g·L-1（NO.34）; The best lateral buds germination medium of ‘Fenpan cang zhu’ is1/2MS+GA31.5mg·L-1+6-BA1.0mg·L-1+AC1.0g·L-1（NO.47）; The best lateral budsgermination medium of ‘Qi hua lu shuang’ is1/2MS+GA31.5mg·L-1+6-BA0.5mg·L-1+AC1.0g·L-1（NO.46）; The best lateral buds germination medium of ‘Dan feng’ is1/2MS+GA31.5mg·L-1+6-BA1.5mg·L-1+AC1.0g·L-1（NO.48）；The best cluster buds induction culture medium of ‘Luhong’ is1/2MS+GA32.0mg·L-1+6-BA2.0mg·L-1+AC1.0g·L-1.③The way and method of root inductionTake the bulbil of ‘Dan feng’ to rooting induction, longitudinal shoot-split method and quicklydip method are all failed to get root; Plantlets take root is difficult, there were few root formation onthe medium1/2MS+IBA0.5mg·L-1（NO.53）; There were less root formation on lateral budgermination medium, when the base of buds exposed to the air. Maybe peony root formation needsexcellent expansibility medium.（4） Obtained the callus from different explants and explored the callus proliferation issuesStem, leaf, cotyledon, root were taken as explants for callus induction. The best callus inductionmedium of stem section is MS+6-BA1.00mg·L-1+NAA0.5mg·L-1（NO.60）; The best callusinduction medium of blade and cotyledon is MS+6-BA2.0mg·L-1+NAA0.50mg·L-1（NO.66）.Callusproliferation with serious Browning, did not obtain ideal proliferation. |
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