The Effect Of Electroacupuncture On The Relative Protein Expression In Nonalcoholic Fatty Liver Disease Rats

BACKGROUNDNonalcoholic fatty liver disease has become the second to the viralhepatitis liver disease, with the changes in the structure of diet, lifestylechanges and pressures are closely related. Western medicine thinks,nonalcoholic fatty liver disease is a kind of accumulation in the liver tissuehad no history of heavy drinking a lot of fat (mostly triglycerides). Livercells significantly fatty degeneration of fat metabolism disorders, itspathogenesis is not yet completely clear. Traditional Chinese medicinethinks, nonalcoholic fatty liver disease belongs to Chinese medicine"obesity, phlegm retention, accumulation" category. Due to overeatinggreasy food, feel dampness and heat, emotional disorder pathogenic, withphlegm and blood stasis, imbalance of liver and spleen. At present,western medicine has not been found the effects of the treatment ofnonalcoholic fatty liver disease drugs and treatment the effect not beautiful.Therefore, research on nonalcoholic fatty liver disease from theperspective of Chinese medicine, using electroacupuncture in the treatment of nonalcoholic fatty liver disease has important clinical value.ObjectThis paper aims to study the effect of Electroacupuncture onnonalcoholic fatty liver rats effects of eliminating blood stasis,harmonizing liver and spleen and regulating lipid metabolism, and theprevious experimental results, to provide the experimental basis for thedevelopment of acupuncture is an effective, safe treatment of nonalcoholicfatty liver disease. At the same time create a new way of thinking for thetreatment of nonalcoholic fatty liver disease. This experiment, from theperspective of the gene and protein expression, cell signal transduction forlater research studies provide preliminary experimental basis.Methods44female SD rats were fed for7days to adapt the environment andwere randomly divided into normal group, model group, Dongbaogantaigroup, electroacupuncture group, with11rats in each group. Normalgroup got routine feeding, and the others were fed with high fat and highcholesterol forage (2%cholesterol,10%lard, and88%basic forage). After8weeks, the models were established, the rats in the electroacupuncturegroup (1.5-3Hz, D.-D.wave,9v,1-3mA) were treated withelectroacupuncture method conbining “Ganshu”,“Pishu”,“Geshu” for15min, once daily, continuously for28days. Method for the colorimetricdetermination of liver homogenate malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), triglyceride (TC), total cholesterol(TG) content; enzyme linked immunosorbent assay (ELISA) fordetermination of serum retinol binding protein4(RBP4) content,Immunohistochemical method liver tissue Fas, FasL, the expression ofcytokeratin18(CK18) protein, By real-time fluorescence quantitativemethod (rt-pcr) determination of cysteine protease8(Caspase-8) proteinexpression level and protein determination method of western blot (WB)peroxidase body proliferation activators receptor alpha (PPAR alpha),cholesterol, regulating element binding protein1c (SREBP-1c), liver Xreceptor alpha (LXR-α) protein expression level.Results:The effects on liver function: Compared with the normal group, modelgroup rats weight gain, depression, less activity, liver color yellow, havegreasy feeling. Model group in the rat liver homogenate SOD and GSHcontent significantly decreased (P <0.01), MDA, TC, TG contentincreased significantly (P <0.01), Compared with the model group,electroacupuncture group rats increase and Dongbaogantai group rat liverhomogenate SOD, GSH content (P<0.01), MDA, TC, TG contentdecreased significantly (P<0.01), Electroacupuncture group was betterthan the Dongbaogantai group (p<0.01or p<0.05).Electroacupuncture stimulation "liver Shu","spleen","Geshu" on nonalcoholic fatty liver rat liver function improvement and promote. Influence on liver lipid metabolism: Compared with the normal group,the content of RBP4in serum in the rats of model group increasedsignificantly (P<0.01), decreased PPAR protein expression in liver tissue(P<0.01), LXR-α and SREBP-1c protein expression level increased(P<0.01), Compared with the model group, electroacupuncture groupdecreased and Dongbaogantai group rats serum RBP4levels, increasedPPAR protein expression in liver tissue (P<0.01), the expression of LXR-α and SREBP-1c protein level decreased (P<0.01or P<0.05),electroacupuncture group is better than the Dongbaogantai group.Electroacupuncture and Dongbao Gantai contributes to improve the nonalcoholic fatty liver in rats with fatty acid and cholesterol metabolism,electro acupuncture is better than that of Dongbaogantai.Electroacupuncture and Dongbaogantai contributes to improve the nonalcoholic fatty liver in rats with fatty acid and cholesterol metabolism,electroacupuncture is better than that of Dongbaogantai.On the repair of liver injury: Compared with the normal group,increased Fas, FasL expression in liver tissue of the rats in the modelgroup (P<0.05), relative expression of caspase-8RNA decreased (P<0.05),Compared with the model group, electroacupuncture group decreasedexpression of rat and Dongbaogantai group rats liver tissue Fas, FasL(P<0.05), the relative expression level of caspase-8RNA increased(P<0.05), electroacupuncture group is better than the Dongbaogantai group. Electroacupuncture and Dongbao Tech Repair of liver in rats withnonalcoholic fatty liver damage liver cells, electro acupuncture is betterthan that of Dongbao gantai.Conclusion:Electroacupuncture "GanShu","PiShu","Geshu" can improve the nonalcoholic fatty liver rat liver function, improve the accumulation of fat,regulating the liver metabolism, repair the damaged liver tissue. Itsmechanism may be related to regulating the liver metabolism function,change the function of protein expression.