Correlation Study Between Small Molecule Metabolites And Type2Diabetes Mellitus In Uygur And Kazak Population In Xinjiang

Objective:To investigate the content variation of uric Acid, creatinine and amino acid among Uygur and Kazak diabetes population with and without diabetes in Xinjiang and study the correlation between Small Molecule Metabolites in Body Fluids and Type2Diabetes Mellitus, thus providing a new perspective and experimental basis for comprehensively comprehending the differences and links in terms of pathogenesis of diabetes. Methods:1. HPLC method:the urine samples were assayed by RP-HPLC on Hypersil ODS C18column,(250mm×4.6mm,5μ m) at the ambient temperature and a wave length of235nm, using a20mmol/L pH6.5ammonium acetate buffer as mobile phase with the flow rate of1.0mL/min to determine the contents of such Small Molecule Metabolites as uric Acid and creatinine;2.AQC method:The determination was performed on waters e2695system, using a Shim-vp, ODS column with mobile phases of methanol/acetate/H2O and sodium acetate buffer(pH6.5), gradient elution,the content of amino acid were testing by Fluorescence detector at a wavelength of395nm by AQC reagents.Result:1.The comparison between Uygur and Kazak groups:The content of blood uric acid in Uygur NG Group is markedly higher than in Uygur DM group(P<0.01). The content of blood uric acid in Kazak DM Group is markedly higher than in Kazak NG group(P<0.01). The content of blood creatinine in both Uygur and Kazak DM Group is markedly higher than in both Uygur and Kazak NG group(P<0.01). The content of uric acid of urine in Uygur DM Group is markedly higher than in Uygur NG group(P<0.01). Comparison between Uygur and Kazak groups:The content of blood uric acid is markedly higher in NG Group of Uygur than that in Kazak NG group(P<0.01). The content of blood creatinine is markedly higher in Uygur NG Group than in Kazak NG group(P<0.01). The content of uric acid of urine in Uygur IFG Group is markedly higher than in Kazak IFG group. In terms of the content of urine creatinine, there is no marked difference between the two groups.2. Metabolite profiles in the urine and serum were analysised by HPLC method.Than,a multivariate analysis and one-dimensional statistical analysis were used to analysis the result,The serum amino acid metabolism including aspartate, glutamic, serine, threonine are found as the most obvious changed amino acid between diabetes and normal group by multivariate analysis. Aspartic acid and phenylalanine metabolic is most significantly increased in diabetes group than in normal group (P<0.01), and alanine, glycine, lysine (P<0.01) and glutamate (P<0.05)is decreased significantly in diabetes than in the normal group by one-way ANOVA, In urine metabolism, the result shows that serine, threonine were most contributed to inter-group, and one-dimensional statistical analysis showed that the urine arginine and histidine and serine significantly decreased significantly (P<0.05), glutamic acid increased significantly (P<0.05) in diabetes than in normal group. Conclusion: There were significantly difference in Uric Acid acid and Creatinine concentration in urine and serum of Uygur and Kazak participant in Xinjiang. The serum and urine amino acid metabolism profiles in Uygur were obviously changed in diabetes and normal,although the difference is not the same amino acids in serum and urine. We suppose that Serum phenylalanine, alanine, lysine, aspartic acid and glycine might be the potential metabolic markers to differentiate diabetes and normal ones and can be seen as potential biomarkers.