Studies On Quality Standards Of Brassica Rapa L.Seed

Objective:In this study, according to the Chinese Pharmacopoeia quality standards as the guiding principle, using a variety of techniques and methods, the establishment of sub-microscopic identification, TLC, conventional project inspection, determination of the chemical composition and fingerprints, on this basis, the author of Brassica rapa L.Seed the quality of the draft standard. Method:1. The use of sub-microscopic features of the Brassica rapa L. Seed powder were identified.2. Using TLC method established quercetin, kaempferol and isothiocyanate.3. Inspected multiple batches of Brassica rapa LSeed moisture, ash, acid insoluble ash and alcohol-soluble extract routine program.4. To establish the method for the determination of the chemical composition of Brassica rapa L.Seed by HPLC method. Using a measured multi-rating extrapolate the content of the sample. To establish the fingerprint of Brassica rapa L.Seed by GC-MS and HPLC method.5. UV method and TLC bioautography research antioxidant activity. Result:1. Microscopic characteristics of the Brassica rapa L.Seed powder containing starch grains, seed coat palisade cells, seed coat epidermal cells, seed subcutaneous skin cells, endosperm cells, cotyledon cells and square crystal.2. TLC screened optimum chromatographic conditions quercetin, kaempferol and isothiocyanates well separated in the sample to be identified.3.10 batches Brassica rapa LSeed moisture content between 4.50%～6.50%; total ash content between 4.30%～6.40%; acid insoluble ash content between 0.30%-1.20%; alcohol-soluble extract measurement results at 13.10%-26.80% between.4. HPLC determination method, after methodological study showed that quercetin is not less than 13μg/g, kaempferol not less than 21μg/g. External standard method with a measured multi-rating for the determination of three isothiocyanates no significant difference in ester content, allyl isothiocyanate is not less than 11μg/g, and isopropyl isothiocyanate not less than 3μg/g, phenethyl isothiocyanate not less than 7μg/g. GC-MS and HPLC fingerprints meet the technical requirements.5. Anti-oxidation activity assay results showed that the IC50 of the DPPH, OH-,02·- were 12.78mg/mL, 38.25mg/mL,32.72mg/mL. By TLC bioautography, quercetin and kaempferol were identified, indicating Brassica rapa L.Seed have antioxidant activity. Conclusion:1. In this study, Chinese Pharmacopoeia quality standards as the guiding principle, the establishment of a sub-microscopic characteristics, methods TLC, routine inspection project, determination, etc. Write draft quality standards of Brassica rapa LSeed.2. A measured multi-rating method content of the sample calculated and measured results of the external standard method content or less, can be used in reference shortage of content and index components measured, especially for multi-index for evaluating the quality of traditional Chinese medicine.3. The use of Bio-TLC method, not only can be used to confirm whether the antioxidant activity and antioxidant active ingredients can be identified, the method is simple and feasible.