The Extract Technology And Quality Standard Of Rhodiola

Rhodiola for sedum Rhodiola Rhodiola crenulata(Hook.f.et Thoms.) H. Ohba dried root and rhizome, with Qi and blood, all child asthma efficacy for blood stasis, chest pain, stroke, paralysis, fatigue, shortness of breath, now commonly used in traditional Chinese medicine as contained in a 2010 edition of "Chinese Pharmacopoeia."This paper studies the Salidroside tannin extract and process optimization in place processes and quality control of its specific contents and results are as follows:1. Salidroside establish measurement methodThe experimental method for the determination of Rhodiola, first Salidroside standard UV scanning to determine the maximum absorption wavelength; then examines Salidroside linear relationship, the results show Salidroside at 10 ~ 200μg / ml concentration range and peak area showed a good linear relationship, precision, stability, repeatability and accuracy are in line with regulations. The results show that the method is accurate, simple, fast, can be used Rhodiola salidroside content.2. Study on the extraction of salidrosideSalidroside Rhodiola is recognized appraisal value of herbal medicine with the most important indicators. The experiments were optimized alcohol extraction and water extraction process Salidroside results showed that the optimum water extraction Salidroside conditions: Rhodiola 40 mesh particle size, solid-liquid ratio 1g: 20 mL, 600 W microwave treatment 20 s, 80 ℃ heat extraction 1.5h, extraction rate of 0.733%. Salidroside alcohol extraction process conditions: Rhodiola size of 40 mesh, 80% ethanol as solvent, solid-liquid ratio 1g: 20 mL, 70 ℃ extraction 2h, the extraction rate of 0.623%. The test proved that the improved water extraction method to extract better than alcohol.3. Rhodiola tannin establish measurement methodThis experiment established the method for the determination of tannin content. First of gallic acid as standard were out scans to determine the maximum absorption wavelength; and gallic acid as the reference, phosphomolybdic acid as a chromogenic reagent, casein as adsorbent, using spectrophotometric absorbance was measured, the results indicate that gallic acid at a concentration of 2 ~ 10μg / ml, and the absorbance in the range of a good linear relationship, precision, stability, repeatability and accuracy are in line with regulations. The results show that the method is accurate, simple, fast, can be used Rhodiola extract tannin content determination.4. Removal Process Rhodiola TanninsThe experiments were investigated gelatin precipitation and removal of cyclodextrin embedding Rhodiola extract tannin effect, and optimize embedded technology cyclodextrin treatment Rhodiola extract tannin process conditions. The results show that optimum conditions cyclodextrin embedding technique is treated Tannins 1L Rhodiola extract added α-cyclodextrin 0.7g, β- cyclodextrin 2.8g, 200 r / min stirring, 50 ℃ heating for 3h. Using cyclodextrin embedding technology can well remove the Rhodiola extract tannin extract well preserved while the salidroside.5. Rhodiola extract quality standardsIn this study, the quality control methods Rhodiola extract, using high-performance liquid chromatography of salidroside extract, the extract was determined by spectrophotometry of tannin, while part of the extract heavy metals were determined. After determination of Rhodiola extract of salidroside content 7.24 mg / g, tannin content was 5.08 mg / g; heavy metals copper, cadmium, lead, arsenic, mercury levels were less than 1.0ug / g, were compliance; by heavy metal detection, determination to have good control of the quality of Rhodiola extract, and lay the foundation for further study of Rhodiola.