The Molding Process And Quality Standard Of Pongamia Pinnata Flavonoids Capsules And ITS Pharmacodynamic Study

Objective:On the basic research of the extraction and purification process of total flavonoids from Pongamia pinnata,explore the molding process and quality standard of its flavonoids capsules and study on its anti-inflammatory effects and antithrombotic effects.Methods:(1) Angle of repose,moisture absorption rate,critical relative humidity and bulk density were used as index to determine the composition of Pongamia pinnata flavonoids capsules.(2) TLC was used for qualitative identification of Pongamia pinnata;UV and HPLC were adopted to determine the content of flavonoids and karanjin.(3) The inflammation model were established by cotton pellet granuloma,mouse ear edema, peritoneal capillary permeability and rat pedal swelling.The pathological changes of rat paw inflammatory tissue were observed under the light microscope and the contents of IL-1β,TNF-a and PGE2 in rat right rear toe tissue were measured by ELISA kits.(4) Three experimental models,including mouse tail bleeding model,mouse tail thrombosis model and inferior vena cava thrombosis model in rats were used to examine the antithrombotic effects of Pongamia pinnata flavonoids capsules in normal and thrombosis rats and mice.The pathological changes of inferior vena cava tissue were observed under the light microscope.The prothrombin time (PT) and activated partial thromboplastin time (APTT) were measured by blood coagulation analyzer and the contents of t-PA and PAI-1 in rats plasma were measured by ELISA kits to evaluate the effects of Pongamia pinnata flavonoids capsules on the anticoagulant factor and fibrinolytic system.Results:(1)10.0% micro powder silica gel was selected as the best prescription form and CRH was 63.7%.0# vacant capsule could meet the requirements of capsules filled.(2)TLC plates were clear without reference and the content of flavonoids should not less than 162.42 mgand karanjin should not less than 39.56 mg in each capsule.(3) Pongamia pinnata flavonoids capsules and PRF showed significant anti-inflammatory effects and could inhibit the release of IL-1β,TNF-a and PGE2. And there were no difference between the same dose of Pongamia capsules and PRF.(4) The bleeding time, prothrombin time and activated partial thromboplastin time were significantly prolonged.The black tail length and the thrombus wet weight were significantly reduced with Pongamia pinnata flavonoids capsules.In addition,it could promote the release of t-PA and inhibit the release of PAI-1.Conclusion:(1)Determined the composition of Pongamia pinnata flavonoids capsules.(2)The qualitative identification methods of TLC and the determination methods of total flavonoids and karanjin by UV and HPLC of Pongamia pinnata flavonoids capsules were established. The quality control method is specific and reproducible, which could be adopted to quality control of Pongamia pinnata flavonoids capsules.(3) The anti-inflammatory effects did not reduce when PRF made into capsules, and it may by through reducing IL-1β,TNF-a and PGE2 levels to achieve the effects.(4)There is a significant antithrombotic effects about Pongamia pinnata flavonoids capsules and its mechanism may be related to increasing the activity of the anticoagulant factor and fibrino lysis system.