Research Of IL-28B Single Nucleotid Polymorphism In The Distribution And Expression Of Patient With Chronic Hepatitis C

ObjectiveTo investigate the distribution of IL-28B single nucleotide polymorphism and explore the dynamic change of interleukin-28B during combinating interferon and ribavirin therapy in patients with chronic hepatitis C.MethodsDatas and anticoagulant whole blood of 60 cases of patients with chronic hepatitis C were collected. IL-28B single nucleotide polymorphisms (SNPs) of rs8099917,rs 12980275 and rs12979860 were tested by multiple high temperature ligase detection; 146 cases of serum samples were collected, including 46 cases of chronic hepatitis C before treatment and 44 cases of them after 12 weeks of therapy and 11 cases of type 1 b at 48 weeks, 33 cases of type non-1b at 24 weeks,12 cases of healthy controls. The serum of interleukin-28B were examined by enzyme-linked immunosorbent assay (ELISA) and the correlation of interleukin-28B with clinical parameter was analysed.Results(1) The percentage of patients in non-genotype 1b was 71.7% (43/60), genotype 1b accounted for 28.3% (17/60). IL-28B SNPs of rs8099917 TT, rs 12980275 AA, rs 12979860 CC was 80% (48/60) in patients with chronic hepatitis C. the IL-28B SNPs of rs8099917 GT, rs12980275 GA, rsl2979860 CT was 58.8%(10/17) in patients with genotype 1b. Statistically significant distribution were found in IL-28B SNPs between genotype 1b and non-genotype 1b (P=0.000). (2) There were no significant difference with aspartate aminotransferase (AST), alanine aminotransferase (ALT), γ-glutamyl transferase (GGT) and viral load in the TT/AA/CC and GT/GA/CT genotype group. (3) Rs8099917 TT genotype and rs8099917 GT genotype rate were 87.0% (40/46) and 13.0% (6/46) among the 46 cases of patients with chronic hepatitis C. The serum of interleukin-28B in patients with chronic hepatitis C and healthy group, hepatitis C viral genotype 1b and non-1b group, high viral load and low viral load group, rs8099917 TT genotype and GT genotype group had no statistical significance (P>0.05). There were no correlation between serum interleukin-28B and aspartate aminotransferase(AST), alanine aminotransferase(ALT), y-glutamyl transferase (GGT), platelet(PLT). (4) The serum of interleukin-28B was higher in early virological response group which included 20 cases than non early virological response group which included 24 cases, the difference was statistically significant (p=0.000);At the end of treatment,30 cases achieved end treatment virological response, their serum interleukin -28B were higher than non end treatment virological response group of 14 cases, statistically significant difference was found(p=0.049); Among 21 cases achieved sustained virological response, their serum of interleukin-28B were higher at 12 weeks and the end of treatment than non sustained virological response group of 23 cases, the difference was statistically significant (p<0.05).ConclusionHCV genotype non-lb of CHC patients mostly carried rs8099917 TT, rs12980275 AA, rs12979860 CC genotype. The IL-28B SNPs of rs8099917, rs12980275, rs12979860 was strong linkage disequilibrium. The rs8099917 genotypes of patients with chronic hepatitis C are mainly composed by TT genotype in Chongqing area. There is no significant difference in the levels of interleukin-28B between rs8099917 TT genotype group and rs8099917 GT genotype group. Serum interleukin-28B seemed to eliminate viruses cooperated with I type of interferon in patients with chronic hepatitis C.