Research Of Interleukin-37 For Inhibits Murine Lewis Lung Carcinoma Cell Proliferation

Introduction:IL-37 was discovered in Silico research of human databases which was formerly termed IL-1 family member 7, and also defined as IL-IF7/IL-1H4. However, human still poorly understood the role of IL-37. More studies about IL-37 can helps to strengthen us understanding with autoimmune diseases, inflammatory diseases and cancer awareness, and to provide a new target for the treatment of malignant tumor. Lymphocytes plays a very important role in the body's anti-tumor immune response, such as killing tumor cells by direct or indirect role in cell-mediated immunity and humoral immunity. Additionally several studies have shown that IL-37 inhibited solid tumors.Therefore, The aims of the present study was to explore the role of IL-37 in T cell immune responses by detecting exogenous IL-37 on T cell activation and proliferation, and to observe its effect on the pathological process of Lung cancer in mice, and to explore its possible mechanism by transfecting recombinant human IL-37 adenovirus into Lung cancer cell lines LLC. Method:Transfer of IL-37 Gene into LLC and detection of IL-37 secretion by ELISA and Western bolt. Establishment of the LLC lung cancer model in mice, Evaluation of the prevalence of mice by detection tumor size and weight. The influence of IL-37 for T cell in vitro were tested. Results:From the results of fluorescence microscopy and flow cytometry results, Adv-IL-37 transfected LLC MOI equal to 100, 80% transfection efficiency, and does not affect cell normal growth state, LLC-Adv-IL-37 cells and normal secretion of IL-37 gene expression and protein. High expressionof hIL-37 adenovirus transfected LLC. There was no significant difference of the self-renew capacity among LLC, LLC-Adv-IL-37 and LLC-Adv-eGFP. IL-37 inhibited the development of Lewis lung cancer in mice: LLC-Adv-IL-37 cells, LLC-Adv-eGFP cells and LLC cells were inoculated into micecompared with the model group and the control group, with significant difference P<0.01, and high survival. but for nude mice cells is not obvious. IL-37 can promote CD4+ T cell and CD8+ T cell activation: on T cell function from different sources differ, such as the spleen lymphocytes without significantly promote the proliferation of sorting CD4+ T cells and CD8+ T cell can promote, make the CD4+ T cells and CD8+ T cell FSC increase large increase in fissile CFSE peaks. Conclusion:Adv-IL-37 in vitro can effectively transfected LLC cells. Adv-IL-37 had no effect on the proliferation of LLC cells. IL-37 can effectively inhibit the development of Lungt cancer tumors, preventing tumor growth and can improve immunity. IL-37 Can inhibit tumor growth significantly and can enhance organismal immunologic system markedly.