Effects Of Leukemia Inhibitory Factor On Endogenous Neural Stem Cell Proliferation And Glycoprotein-130 Expression In A Mouse Model Of Cerebral Infarction

Object:To detect the GP130 protein expression and the proliferation of ENSCs in the mouse model of CI after LIF administration.Methods:Thirty-two mice,aged 8 weeks,weighing 18-20g,were divided into four groups:Sham Group(SHA),Contrary Group(CON),Cerebral Infarction Group(CI)and LIF-treated Group(LIF),with equal numbers of males and females.These groups were ended at the third week after the beginning of the experiment.Sixteen mice were used to detect the number of ENSCs,while the other sixteen were used to study the experiment of GP130 protein.1.Mice in all groups accepted neuro-function tests:Rotarod Test,the data was statistically analyzed.2.The number and proliferation of ENSCs in the mouse brain after CI were studied using immunohistochemistry method before and after LIF administration.3.The relative expression of GP130 protein surrounding the infact foci was detected with Western blot.Results:1.Moter function detection:At 0 week(immediately)after model establishment,all mice could stay on the Rotarod for the maximum testing time of 180 seconds;at 1,2,and 3 weeks,the stay time remained unchanged in the sham-surgery and control groups,but the stay time of the model and LIF groups was significantly decreased compared with the sham-surgery and control groups or the 0 week time point(P<0.05).Moreover,the stay time was prolonged in the LIF group compared with the model group at 2 and 3 weeks(P<0.05).2.Number of ENSCs:At 0 week after model establishment,ENSCs were not activated in any of the groups.At 3 weeks,no activated ENSCs were observed in the sham-surgery and control groups,but ENSCs were significantly activated in the model and LIF groups compared with the 0 week time point(P<0.01).Moreover,the LIF group exhibited more activated ENSCs than the model group(P<0.01).3.Expression of GP130 protein:At 3 weeks after model establishment,GP130 protein expression was significantly elevated in the model and LIF groups compared with the sham-surgery and control groups(P<0.05).Moreover,gp130 protein expression was significantly decreased in the LIF group compared with the model group(P<0.05).Conclusion:LIF plays a role in prohibiting the expression of GP130 protein and promoting the proliferation of ENSCs.The potential mechanisms of the finding in both GP130 protein and ENSCs need to be further investigated.