Effects Of Positive Acceleration (+G_Z) On Oxidative Stress Of Renal Calcium Oxalate Calculus Rat Models

Objective The incidence of kidney stones in the pilots is high.To investigate the effects of positive acceleration(+G_Z)on oxidative stress of renal calcium oxalate(CaO_x)Calculus rat models.Methods The model of renal CaO_x was established with ethylene glycol(EG)in drinking water and ammonium chloride(AC)by garage.A total of 40 adult healthy male Wistar strain rats were randomly and averagely divided into 4 groups(10 of each): blank control group(A),stone forming group(B),stone forming with +Gz exposure group(C)and + GZ exposure naive group(D).Group A was administered with drinking water and physiological saline by gavage(2ml/d);group B was administered with 1% EG in drinking water and 2% AC by garage(2ml/d);group C,administered with 1% EG in drinking water and 2% AC by garage(2ml/d),was exposed to +6G;Group D,administered with drinking water and physiological saline by gavage(2ml/d),was exposed in +6G.After feeding for 28 days,blood serum and renal samples were collected.The presence of CaO_x crystals was scored in left renal under hematoxylin and eosin(HE)staining,and the level of malonddialdehyde(MDA),superoxide dismutase(SOD),catalase(CAT),glutathione(GSH)and glutathione Peroxidase(GSH-Px)in the blood serum and right renal homogenate were measured as indicators of oxidative stress.Results For the formation of CaO_x crystals,the scores of group B and group C were significantly higher than group A and group D(P<0.01),the scores of group C were significantly higher than group B(P<0.01).For the parameters of oxidative stress,compared with group A,the concentration of MDA in blood serum and kidney tissues of group B significantly increased(P<0.01),which significantly increased(P<0.01)after the intervention of +G_Z(group C).Compared with group A,the content of SOD,CAT and GSH-Px in blood serum and kidney tissues of group B significantly decreased(P<0.01),which significantly increased(P<0.01)after the intervention of +G_Z(group C).However,the +G_Z showed no significantly influence either GSH in blood serum and renal homogenate(P>0.05).After HE staining,the sections were observed under microscope by three experienced pathologists.The group A: there was no calcium oxalate crystal found in renal tissue,and the structures of renal papilla,renal pelvis,renal calyx were normal.The group B: there was a small amount of calcium oxalate crystal found in renal papilla,renal pelvis and renal calyx,and a small amount of inflammatory cell infiltrated in the renal tissue,and epithelial cells can sometimes be seen swelling.The group C: there was a large number of calcium oxalate crystal found in renal papilla,renal pelvis and renal calyx,and a great deal of inflammatory cell infiltrated in the renal tissue,and epithelial cells can be seen swelling.The group D: it is similar to group A in the aspect of calcium oxalate crystal,but there are a small amount of inflammatory cells infiltrated in the renal tissue.Conclusion positive acceleration(+G_Z)might be one of the risk factors contributed to kidney stone formation,The formation of rat renal calculus might be correlated to the oxidative stress damage of renal tissue.