Protective Effects And Mechanism Of Quercetin On Astrocytes Cells Induced By Hypoxia/Hypoglycemia And Reperfusion Injury

Objective:To investigate the effect of quercetin(QUE) on hypoxia/hypoglycemia and r eperfusion(H/R) injury of SD rat astrocytes, and reveal the possiable mechanism of this e ffect.Methods:Perpare the primary cultures of astrocytes cells.Na2S2O4 induced astrocttes in jury to establish in vitro H/R model. Cells were divided into control grop, OGD/R model grop, nimodipine grop and quercetin grop(10,1and0.1μmol/l). The cell viability was assesse d with CCK-8 assay and the LDH release late. The PI/Hoechst33342 staining observation was used to determine the apoptosis morphology and characteristics after H/R injury. Wes tern blottingwas used to detect the expression of GLT-1. The change of mitochondrial me mbrane poten-tial was measured by Rh123 staining method. SOD, GSH, MDA and ROS a ssay kits were used to determine the activity of SOD, GSH, MDA and ROS. Western blot ting was used to detect the expression of Bax, Bcl-2, Caspase-3 as well. Perpare the ne uronal cells which were isolated from SD rats cortex at dayResults: 1. It is successed to culture brain cells of astrocytes and neurons. The best condition was found to establish the hypoxia/hypoglycemia and reperfusion injuried model.2. MTT and LDH results showed that QUE could improve the survival rate of astrocytes after hypoxia/hypoglycemia and reperfusion injury. QUE pretreatment can obviously impro ve the apoptotic morphology of injured astrocytes, and reduce the rate of apoptosis of astr ocytes after H/R injury. 3. QUE treatment increased GLT-1 protein expression and MMP i n astrocytes. SOD and GSH assay kits results showed that QUE could improve the activity of SOD and GSH in astrocytes after H/R injury. QUE could reduce the activity of M DA and ROS. WB results showed that QUE could improve the expression of Bcl-2 protei n and reduce the expression of Bax and Caspase-3 protein in astrocytes after H/R injury.Conclusions: QUE has strong cytoprotection to astrocytes after H/R injury.The mecha nism of action very probably is that QUE could improve the expression of GLT-1 protein.Then the MMP was reduced, Oxygen free radical aggregation was cut down, apoptotic g enes were suppressed.