Experimental And Clinical Study On Treating Qi-stagnation Indueted Blood-stasis Angina Pectoris Of CHD With Xinkeshu

Objective: From two aspects of animal experiment and clinical research, to observe the effect of Xinkeshu(XKS) on the clinical efficacy and security,and analysis its mechanism of anti-atherosclerosis..(1) Animal experimentsMethods: divide New Zealand rabbits into four groups by random :normal control group, high-fat group, fluvastatin group and Xinkeshu(XKS) group(9 /per group). The atherosclerotic plaques were evaluated by HE staining. Expression of MMP-9,macrophage andα-SMA in atherosclerotic plaques were detected by immunohistochemistry.Results: 1) The high fat group, Xinkeshu group and fluvastatin group both have atherosclerotic plaque formation.The high fat group was obvious. Xinkeshu group and fluvastatin group had less smaller plaque formation than high fat group. The control group had no plaque formation. 2)Compared with high-fat group,the expressions of VCAM-1 in atherosclerotic plaques significantly decreased with the treatment of Xinkeshu(P<0.01).3)The expression of MMP-9, macrophage infiltration andα-SMA were also lower in the XKS group than that in high-fat group(P<0.01).(2)Clinical trialsMethods:according to the cases into the standard, a total of 60 patients with Qi-stagnation indueted blood-stasis stable angina pectoris of coronary heart disease(CHD),choosing from department of traditional Chinese medicine and cardiology outpatients of the Provincial Hospital Affiliated to Shandong Univeristy,were randomly divided into 2 groups. 30 cases in control group, only were treated with basic treatment and30 cases in treatment groupas were treated with basic treatment and Xinkeshu tablet 2#, Tid.4weeks later, the clinical curative effect evaluation. Respectively on two groups of patients with the degree of angina pectoris,TCM syndrome, electrocardiogram and safety test wereobserved and compared to evaluate the efficacy and safety.Results: 1) Clinical research confirms,in treatment of TCM syndrome integral, the treatment group had obvious improvement, and better than the control group,(P<0.01). 2)In the treatment group, angina pectoris frequency,lasting time,the dosage of nitroglycerin,ECG manifestations and traditional Chinese medicine symptoms curative effect were better than that of the control group(P < 0.05).Conclusion: Xinkeshu in clinical treatment of Qi-stagnation indueted blood-stasis angina pectoris of CHD achieved certain effect, is a safe and effective prescription;Basic researchs confirm that XKS play an important role in the protection of endothelial function and anti-atherosclerosis effect by its anti-inflammatory. In a word, XKS can be effective in the treatment of stable angina pectoris of CHD.