Study On Effect Of Sargentodoxacuneata Total Phenolic Acids On Cerebral Ischemia Reperfusion Rats And Mice Model

Objective: Study the effect of Sargentodoxacuneata total phenolic acids on cerebral ischemia reperfusion rats and mice model about neuron enolase(NSE),interleukin-6(IL-6),interleukin-10(IL-10),interleukin-1 β(IL-1β),tumor necrosis factor α(TNF-α),intercellular adhesion molecule 1(ICAM-1),Bcl-2 associated X protein(Bax),B cell lymphoma factor 2(Bcl-2),cysteine proteinase 3(Caspase-3),ATP enzyme,superoxide dismutase(SOD),malondialdehyde(MDA)level of observation;nuclear transcription factor(NF-κBp65),nerve growth factor(NGF)expression,and the impact of the meningeal microcirculation,scores of neurological deficit,cerebral infarction area and the morphological changes of brain tissue,so as to explore the protective mechanism of Sargentodoxacuneata total phenolic acids on cerebral ischemia reperfusion injury.Methods: in mice with repeated cerebral ischemia again perfusion model by blocking bilateral common carotid artery to the blood flow in ten minutes,to restore perfusion for ten minutes,re blocking the ten minutes.Nimodipine group,brain collaterals group,large,medium and small dose of Sargentodoxa cuneata total phenolic acid group were given corresponding drugs,sham operation group,model group gavage the same volume of physiological saline,continuous administration for 7 days,1time every day.Operation model for mice after the last administration,the brain tissues were taken after 24 h,and the levels of IL-6,IL-10 and TNF-α in mouse brain were measured.The serum NSE level was measured and the morphological changes of brain tissues were observed.The Perimed instrument was used to observe the changes of cerebral perfusion in mice.Nimodipine group,brain collaterals group,large,medium and small dose of Sargentodoxa cuneata total phenolic acid groupwere given corresponding drugs,sham operation group,model group gavage the same volume of physiological saline,continuous administration for 7 days,1 time every day.After the last administration1 H mice were anaesthetized with 10% chloral hydrate(0.03ml/10g),fixed in the prone position,were observed before and after the mice’s bilateral carotid artery ligation(sham operation group only isolated bilateral common carotid artery,without ligation treatment)average perfusion changes.Establishment of focal cerebral ischemia reperfusion model in rats.Nimodipine group,brain collaterals group,large,medium and small dose of Sargentodoxa cuneata total phenolic acid group were given corresponding drugs,sham operation group,model group gavage the same volume of physiological saline,continuous administration for 7 days,1time every day.Operation model for mice after the last administration,the brain tissues were taken after reperfusion for 22 h,Assessment of neurological deficit scores and the levels of IL-6、IL-1β、TNF-α、Bcl-2 、Bax、Casp-3、ICAM-1、ATP、SOD、MDA in rat brain were measured.The serum NSE level was measured and the morphological changes of brain tissues were observed.The brain tissue TTC staining was performed,the calculated infarct size,brain tissue pathological changes were observed by HE staining,immunohistochemistry of NGF was observed,NF kappa bp65 expression;Results:Successful model of repeated cerebral ischemia reperfusion in mice,experimental results suggest that Sargentodoxa cuneata total phenolic acid in each dose group can reduce the mortality of mice,reduce brain tissue IL-6,TNF-α levels and serum NSE level,Elevated IL-10 level in the brain tissue,improve the brain tissue in the hippocampus,cortical area of pathological injury.Impact experiments of the meningeal microcirculation in mice results suggest Sargentodoxa cuneata total phenolic acid in each dose group can reduce animal meningeal microcirculation mean perfusion decrease in percentage.Successful model of focal cerebral ischemia reperfusion in rats,The results suggest that Sargentodoxa cuneata total phenolic acid in each dose group can reduce the neurological functional deficit scores,mortality rate,the percentage of cerebral infarction area and reduce IL-6,IL-1β,ICAM-1,TNF-α,MDA,Bax,caspase-3 levels in brain tissue,increase SOD,ATP,Bcl-2 in brain tissue,promote the positive expression of NGF in brain tissue,inhibit the expression of NF-KBp65 in brain tissue and improve the pathological damage of hippocampus and cortex in brain tissue.Conclusion: total phenolic acids from Sargentodoxa cuneata can by improving brain tissue energy metabolism,protect brain tissue from injury of inflammatory cytokines and neurotrophic factor increased,protect neurons,reduce apoptosis of nerve cells,activation of brain cells to self protection mechanism,improve the lesions of the cerebral cortex and hippocampus,reduce cerebral infarction,decrease the cerebral ischemia reperfusion injury,play a protective effect on brain tissue.