To Investigate The Expression Of MiR-200b In Tongue Squamous Cell Carcinoma Cell Line And Its Effect On Proliferation And Invasion

Objective: This study aimed to investigate the expression and clinical significance of miR-200 b in human tongue squamous cell carcinoma and its clinical significance.Methods:The different tongue squamous cell carcinoma cells and tongue epithelium cells were cultivated in medium, and the SCC-9, SCC-2, TSCCa and Tca8113 tongue squamous cell lines were respectively divided in B, C, D, E group. The tongue epithelium cells were served as negative control of the experimental groups. Quantitative RT-PCR was performed to detect the expression of miR-200 b in different tongue squamous cell carcinoma cells and tongue epithelium cells. Then 62 cases of tongue squamous cell carcinoma tissues and adjacent normal tissues were collected, the adjacent normal tissues as control group(group A1), tongue squamous cell carcinoma tissues into the experimental group(Group B1), and the group A1 was served as negative control of the group B1. To detect the expression of miR-200 b in the group A1 and group B1 by Quantitative RT-PCR, and to analyze the correlation between the expression of miR-200 b and the clinicopathological data of patients with tongue squamous cell carcinoma. Tca8113 cells were respectively transfected with miR-200 b mimics, scramble, control-si RNA and Bmi-1-si RNA. Among in that scramble(A2 group) and control-si RNA(A3 group) were respectively served as negative control of miR-200 b mimics(B2 group) and Bmi-1-si RNA(B3 group). The protein and m RNA expression of Bmi-1 regulated by miR-200 b was respectively detected by Western blot and q RT-PCR. MTT were employed to detect the proliferation ability of Tca8113 cells.,and transwell invasion assays were employed to detect the invasion ability of Tca8113 cells. Results: q RT-PCR assay showed that miR-200 b was significantly down-regulated in tongue squamous cell carcinoma cells(SCC-9?SCC-2?TSCCa and Tca8113) compared to normal tongue epithelium cells(TEC)(P<0.05).The expression of miR-200 b were down-regulated in tongue squamous cell carcinoma tissues compared with adjacent normal tissues(P<0.05).The expression of miR-200 b was negatively correlated with the clinical stage and lymph node metastasis in tongue squamous cell carcinoma. The study showed that a notable reduction of the m RNA and protein level of Bmi-1 in B2?B3 groups compared with A2?A3 groups((P<0.05)). MTT assay showed that the OD values were significantly lower in B2?B3 groups compared with control groups(P<0.05),and transwell invasion assays showed that the number of Tca8113 cells through the basement membrane was respectively decreased in B2?B3 groups, compared with the control groups(P<0.01). Conclusions: The expression level of miR-200 b was down-regulated in tongue squamous cell carcinoma cells and tissues, and the low expression of miR-200 b was a close correlation with lymph node metastasis and clinical stage.Mi R-200 b inhibited the proliferation and migration of tongue squamous cell carcinoma cells by targeting Bmi-1.Conclusions: 1. The expression level of miR-200 b was down-regulated in tongue squamous cell carcinoma cells and tissues, the low expression of 200 b was a close correlation to lymph node metastasis and advanced clinical stage. 2. miR-200 b may inhibit tongue squamous cell carcinoma cells proliferation and migration by targeting Bmi-1.