| Objective : Blood streptococcus bacteriocins of smooth candida bacteria young's modulus ?deformation quantity ?the adhesion force have an impact on.1. The study of candida glabrate bacteria the size of the young's modulus, adhesion force and deformation quantity.2. Respectively under different concentrations of blood streptococcus bacteriocins of candida glabrate bacteria form deformation quantity, the influence of the adhesion force and young's modulus.Methods:1.the blood streptococcus bacteriocins extraction:Extend the blood streptococcus standard strains to recovery.By using the methods of low temperature high speed centrifugal,ultrasonic broken streptococcus sanguis bacteriocin extracted from blood,streptococcus,and then use PBS streptococcus sanguis bacteriocin will be diuted to different concentrations.(0.25/L?0.5g/L?1g/L)2.The cultivation of the candida glabrate:extend the candida glabrate standard strains of revovery.3.By means of atomic force microscope microscopic contact, Using the relative measuring method(Basic value is adjusted for 3.5Mpa),Measured smooth candida bacteria adhesion strength,young's modulus and deformation quantity.4.Different concentrations of streptococcus sanguis bacteriocin effect smooth candida:Will be 0.25/L?0.5g/L?1g/L Concentration of streptococcus sanguis bacteriocin 200 ul,5×106cfu/ml concentration of smooth candida fungus suspension 500 ul Lto join 1ml of Rpmi 1640 medium,the turbine shock blending, Placed in 37? constant temperature incubator,cultivate 12 h after waiting for inspection.5.By means of atomic force microscpe microscopic contact,using the relative meauring method(Basic value is adjusted for 3.5Mpa),Measured streptococcus sanguis basteriocin function after the smooth of candida,adhesion strength,young's modulus valus and deformation quantity change.Results:1.The cultivation of the candida glabrate:Grow pale purple like cheese and smooth surface form samll bacterial colony.2.Normal smooth candida bacteria's deformation quantity70.38±1.31 nm. Average of adhesion force value 7.31±0.53 n N.For the average of young's modulus 7.14±0.39 Mpa.3.Different concentration of streptococcus sanguis bacteriocin effect after smooth candida :Atomic force micoscopy showed smooth candida bacteria as the different degrees of distortion,surface is rough to form the different size of the disk sag.4.Different concentrations of streptococcus sanguis bacteriocin effect after smooth candida :The adhesion strength,young's moduluss deformation quantity respectively change0.25g/Lstreptococcus sanguis bacteriocin after the role candida glatrate bacteria of deformation quantity80.48±7.96 nm.Average of adhesion force value 6.91±0.76 n N.For the average of young's modulus 6.78±0.68Mpa;0.5g/L streptococcus sanguis bacteriocin after the role candida glatrate bacteria of deofrmation quantity 118.18±30.34 nm.Average of adhesion force valus6.42±1.04 n N,For the average of young's modulus 5.94±1.02Mpa;1g/L streptococcus sanguisbacteriocin after the role candida glatrate bacteria of deformation quantity246.55±87.48 nm.Average of adhesion force value 5.61±0.88 n NFor the average of young's modulus4.75±1.28 Mpa.Conculusions:1. Streptococcus sanguis bacteriocin can lead to smooth candida of bacteria,adhesion strength,young's modulus and deformation quantity valus change.2. 0.25 g /L?0.5/L?1g/L concentrations of streptococcus sanguis bacteriocin effect after smooth candida.Among them 1g/1 of blood chain element to the average of bacteria adhesion strength,young's modulus to minimize the agerage,de formation quantity biggest,a significant bacteriostatic effect.The experimental group compared with control group to present a certain difference(P<0.05).3.Blood chain element in 0~1g/L after the concentration range of smooth candida.Bacteria with the increase of blood concentration of chain element type variable also increase adhesion strength decreases;Young's modulus decreases also;Significant differences differences compared with control group(p<0.05). |
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