Study On Anti-tumor Of Nano Selenium Polysaccharides Of Auricularia Auricula

Objective To obtain black fungus polysaccharide, the experiments of optimized liquid fermentation by Response surface method and tank fermentation by single factor were launched. Black fungus polysaccharide were separated out by alcohol precipitation method. Nano selenium polysaccharide of black fungus, also were investigated on the anti-microbial and antitumor research. This experiments lays the foundation for the research of nano selenium polysaccharide of black fungus.Methods The black fungus is the raw material of the experiment, it was cultured on PDA culture in sterile conditions. After cultured, the black fungus was transferred to the liquid medium. Culture conditions were optimized for liquid fermentation by response surface. Tank fermentation conditions were optimized by single factor. The black fungus polysaccharide from the fluid culture was testing by phenol sulfuric acid method. Modified the polysaccharide of black fungus by nano selenium,was represented by using the TEM. We observed and measured the diameter of the particle. Nano selenium polysaccharide of black fungus, also were investigated on the anti-microbial and anti-tumor research. Nano selenium polysaccharide of black fungus was investigated on the anti-tumor research by MTT.Results Black fungus of white filamentous grew well on PDA medium. We obtained suitable medium for the growth of black fungus, Liquid fermentation were optimized of by Response surface method. The optimal conditions of the black fungus polysaccharide from the liquid fermentation is 3% soluble starch, and 0.15% peptone, 4% Pisha pumpkin, 10% Rhodiola. With this conditions, the black fungus polysaccharide content was 0.02451 g/ml. Thoughe the experiment of fermentation tank culture by single factor, we obtained the optimum temperature was 26?, the optimum rotational speed was 120r/min. The black fungus polysaccharide content was the highest with this conditions. The anti-microbial experiments were investigated by using nano selenium polysaccharide of black fungus from experiment obtained. It was showed from this experiment the minimal inhibitory concentration of Staphylococcus aureus is 50mg/ml, inhibition zone diameter is 7.34mm; minimal inhibitory concentrations of Bacillus subtilis is 25 mg / ml, inhibition zone diameter is 6.58mm; minimal inhibitory concentration of escherichia coli is 12.5mg/ml and diameter of antibacterial circle is 7.95 mm, minimal inhibitory concentration of pseudomonas aeruginosa is 50mg/ml and diameter of antibacterial circle is 6.97 mm. Through the anti-tumor experiment of nano selenium polysaccharide of the black fungus, we can obtaine the results: inhibitory rate of 1.5mg/ml was 61.27%, inhibition rate of 1mg/ml was 44.22%, inhibitory rate of 0.5mg/ml was 41.36%, inhibited rate of 0.25mg/ml was 36.75% and inhibition rate of 0.125 mg / ml was 28.96%.Conclusions Though the expriments, we obtained the optimized liquid fermentation condition of polysaccharide black fungus polysaccharide.Under the optimum conditions, the content of black fungus polysaccharide was the maximum value. Meanwhile we obtained for the cultivation of the tank fermentation. Nano selenium polysaccharide of black fungus solution was obtained from the experiment, we can observe the diameter consistent with the nanometer particle size by using the TEM for representation.The nano selenium polysaccharide of black fungus were best effected to escherichia coli through the anti-microbial expriment. The Nano selenium polysaccharides of black fungus had obvious inhibitory effected on tumor growth, with the concentration of polysaccharide increased, the inhibitory effect was the better. We obtained the best concentration was 1.5 mg/ml from the anti-tumor experiment, meanwhile the inhibition rate was 61.28% in this concentration.