Expression Of HSP70 And NF-κB In The Placental Trophoblasts Of Pregnant Women Complicated With Preeclampsia

ObjectiveThis project plans to analyze the placental tissues of the third trimester of pregnancy using immunohistochemistry methods and real-time fluorescence quantitative PCR technique. The aim is to investigate the difference between the expression of HSP70 and NF-κB in the placenta tissues of normal pregnancy and preeclampsia patients, in order to find out the relation between HSP70 and NF-κB. We expect to reveal the roles of HSP70 and NF-κB in the occurrence and development of preeclampsia. MethodsFrom December, 2013 to January, 2015 65 pregnant patients cases are selected in Fujian Medical University affiliated first hospital of Fuzhou, Among those in the control group, there were 40 preeclampsia patients(including 17 mild cases, 23 severe cases) and 25 healthy pregnant women. All cases had neither complications of internal medicines nor other complications of pregnancy(such as placental abruption, placenta previa, premature rupture of membranes, ABO incompatibility, gestational diabetes, gestational intrahepatic cholestasis, etc.). Each was singleton pregnancy. Two pieces of placental tissues about 1cm3 were collected from the pregnant women immediately when the placenta was delivered. The expressions of HSP70 and NF-κB in the placental tissues were then measured using immunohistochemistry methods and real-time fluorescence quantitative PCR technique. Results1. HSP70 immunohistochemical results suggest that HSP70 mainly are located in the syncytiotrophoblast cytoplasm and nuclei are partly colored. The staining degree of the control group, the mild and severe preeclampsia increases gradually. The difference between the mild/severe preeclampsia group and the control group is significant(P<0.01), while there is no statistical difference between the mild and the severe preeclampsia patients(P>0.05);2. The result of HSP70 real-time fluorescence quantitative PCR suggests that the level of the expression increases in mild and severe preeclampsia patients, as compared with the control group, and it also increases when the disease becomes worse. The difference between the mild/severe preeclampsia group and the normal group is statistically significant(P<0.01);3. The immunohistochemical result of NF-κB p65 shows that NF-κB p65 is mainly located in the syncytiotrophoblast cells, vascular endothelial cell nucleus and cytoplasm of the placenta. The staining degree of the control group, the mild preeclampsia and the severe preeclampsia increases gradually. The difference between the mild/severe preeclampsia group and the normal group is significant(P<0.01), while there is no statistical difference between the mild and the severe preeclampsia patients(P>0.05);4. The real-time fluorescent quantitative PCR result of NF-κB p65 shows that the level of the expression increases in mild and severe preeclampsia patients, as compared with the control group, and it also increases when the disease becomes worse. The difference between the mild/severe preeclampsia group and the normal group is statistically significant(P<0.01);5. The correlation between the expression of HSP70 and NF-κB p65 m RNA shows that HSP70 and NF-κB are correlated with each other, having statistical significance( P<0.01). Conclusion1. The increasing of the expression of HSP70 in the trophoblast cells of pregnant women with preeclampsia, indicates that in the inflammation and hypoxia stress state, the stress expression of HSP70 increases so that the antioxidant stress reaction and the anti-inflammatory ability get enhanced, which help to combat the stress injuries and maintain pregnancy;2. The expression of NF-κB p65 increases in preeclampsia trophoblast cells is an indication that the inflammation in placental tissue and hypoxia becomes more severe than normal pregnancy. Thus, NF-κB may be related to the pathogenesis of preeclampsia eclampsia and clinical symptoms;3. In preeclampsia, the existence of inflammation and hypoxia stress in placenta, leads to the changes in the expression of HSP70 and NF-κB p65. They interact and correlate with each other. This suggests that both of them play an important role in the occurrence and development of preeclampsia.