Analysis Of Clinicopathologic Features And Expression Of POLE In Endometrial Carcinoma

?Background?Endometrial carcinoma is the most common endometrial epithelial malignant tumor. TCGA devided the endometrial cancer into four sgroups according to the molecular phenotype. The POLE mutant,one of the 4 groups, has a unique molecular phenotypic and favorable prognosis. There is no clinical pathological study about POLE immunohistochemical expression pattern research, its expression pattern is still unclear.?Objective?To study the clinical pathology characteristic,immunohistochemical staining, and express the similarities and differences of POLE of endometrioid carcinoma and serous carcinoma.?Methods?148 cases of endometrial carcinomas were collected from January 2006 to June2015 in the Department of Pathology, First Affiliated Hospital of Fujian Medical University. Tissue microarray was made to observe the clinicopathological characteristics of endometrial cancer,and their immunohistochemical expression rate of ER, PR, P53, and P16 and Ki-67, Vimentin and POLE. Detect the gene mutation sites Asp275,Pro286,Ser297,Arg446,Ala456 of POLE and observe the mutations in endometrial carcinoma POLE exonuclease region.?Results?1. According to the morphological and immunohistochemical criteria, 136 cases of endometrial carcinoma and 12 cases of serous carcinoma were diagnosed. Among them, 65 cases were EECs G1, 42 cases were EECs G2, 29 cases were EECs G3.2. The clinical and pathological features of endometrial endometrioid carcinoma are different from the endometrial serous carcinoma.3. The loss or downregulation of POLE expression in endometrioid carcinoma was more significant than endometrial serous carcinoma.4. There was no significant difference in POLE expression between different FIGO grades of endometrial carcinoma.5. No mutation was detected in the five loci Asp275, Pro286, Ser297, Arg446,Ala456 POLE of POLE exonuclease domain in neither endometrioid carcinoma or serous carcinoma.?Conclusion?1. The clinical and pathological characteristics of endometrial carcinoma and endometrial serous carcinoma were different.2. POLE may contribute to the differential diagnosis of endometrioid carcinoma and endometrial serous carcinoma.3. The mutation rate of POLE exonuclease domain was too low,so it can not be used as an independent basis for classification.