Expression And Clinical Significance Of Toll-like Receptor 5 And Toll-like Receptor 9 In Peripheral Blood Mononuclear Cells From Henoch-schonlein Purpura In Children

Objiective To explore the expressions and clinical significance of Toll-like receptor(TLR)5 and TLR9 in peripheral blood mononuclear cells in Henoch-Schonlein purpura(HSP)children, and in order to investigate the roles of TLR5 and TLR9 in the pathogenesis of HSP or Henoch-Schonlein purpura nephritis(HSPN).Methods Eighty-nine children with a clinical first diagnosis of acute Henoch-Schonlein purpura(as group A) were enrolled in the study at Pediatric Department of the MCH of Tangshan city from November 2014 to November 2015. Another 100 healthy gender and age-matched children as controls(group B). Peripheral wenous blood were sampled in aseptic condition for experimental group and control group, peripheral blood mononuclear cell(PBMC) were isolated from density gradient centrifugation. Flow cytometry(FCM)and real-time PCR detected the m RNA and protein expressions of TLR5 and TLR9 in peripheral blood mononuclear cells. While the experimental group to detect urine flow cytometry analysis and urinary micro-albumin. According to their urinary micro-albumin and whether children with HSP had renal damage or not, 89 cases were divided into group A1, A2 and A3. Group A1 were children only with HSP but without renal damage, while group A2 were children only with HSPN not micro-albumin and group C were children with both HSPN and micro-albumin. The Student t test was used for comparison between the HSP patients and the control. The one-way analysis of variance and LSD-t test were used to determine the differencesamong groups. Correlation analysis using Pearson correlation test.Results 1 The mRNA and protein expressions of TLR9 in peripheral blood mononuclear cells were significantly higher in experimental group than those in control group(P<0.05).The m RNA and protein expressions of TLR9 in group A3 were much higher than those in group A1 and A2(P< 0.05). Meanwhile, there was no significant difference between group A1 and A2(P>0.05). 2 Moreover, there was a positive relationship between protein expression of TLR9 and urinary micro-albumin in group A3(r=0.667, P<0.01). 3 The m RNA and protein expressions of TLR5 in peripheral blood mononuclear cells were significantly higher in experimental group than those in control group(P<0.05). But,expression of TLR5 was of no significant difference in group A1, A2 and A3(P>0.05). 4The expression of TLR9 m RNA had a positive correlation with protein expression of TLR9(r=0.689, P<0.01). The expression of TLR5 m RNA had a positive correlation with protein expression of TLR5(r=0.686, P<0.01) too.Conclusions 1 In children with HSP, The expression levels of TLR9 mRNA and protein in PBMCs were remarkably increased;The expression of TLR9 m RNA and protein in PBMCs of children with HSPN showed significantly higher than that of the NSPN group.2 In children with HSP, The expression levels of TLR5 m RNA and protein in PBMCs were increased too. 3 The aberrant activation of TLR5 and TLR9 may be correlated with immunological pathogenesis of HSP.4 Expressions of TLR9 in peripheral blood mononuclear cells significantly increased and had a positive correlation with urinary protein excretion from HSPN in children. This implied that aberrant activation of TLR9 might be relevant to the development of HSPN.