Role Of MiR-23b-3p In 3T3-L1 Preadipocyte Proliferation And Differentiation

micro RNAs(miRNAs) were a class of non-coding small RNA(approximately21-25 nt). Although several mi RNAs had been reported to regulating adipogenesis, the role of mi R-23b-3p on adipocyte diffenentiation was still unknown. The mouse 3T3-L1 preadipocyte was used as the research object to explore the influence of mi R-23b-3p on3T3-L1 preadipocyte proliferation and differentiation. Following the transfection of mi R-23b-3p mimic and inhibitor into 3T3-L1 cells, CCK-8 and flow cytometry were used to measure the effect of mi R-23b-3p on 3T3-L1 preadipocyte proliferation; the expression of cell cycle genes Cyclin D1, CDK4 and adipogenic marker genes PPARγ, FABP4 were detected by real-time q PCR and Western blot; oil red O staining was used to examine the content of triglyceride. The results were as follows:1. Overexpression of mi R-23b-3p inhibited 3T3-L1 preadipocyte proliferation and blocked the G1 phase, the expression of Cyclin D1 and CDK4 were significantly reduced at m RNA and protein levels; inhibition of mi R-23b-3p promoted 3T3-L1 preadipocyte proliferation and decreased the ratio of G1 phase; the expression of Cyclin D1 and CDK4 were upregulated.2. The mi R-23b-3p expression was up-regulated during the differentiation of 3T3-L1 preadipocytes.3. Overexpression of mi R-23b-3p promoted adipogenesis as evidenced by increasing the m RNA and protein expression of adipogenic marker genes, including PPARγ, FABP4,as well as triglyceride accumulation; inhibition of mi R-23b-3p decreased 3T3-L1 differentiation by reducing the expression of PPARγ and FABP4, as well as triglyceride accumulation.Conclusion: mi R-23b-3p inhibits 3T3-L1 preadipocyte proliferation and promotes adipogenic differentiation.