| Objective: Breast cancer is one of the most common malignant tumors in women.The incidence and mortality of the disease are the first in clinic,and this disease seriously harms the health of women.The occurrence and development of breast cancer is a multi factor,multi step complex process,which involves not only the biological characteristics of breast cancer cells,but also closely related to the environment of cancer cell proliferation and invasion.Tumor associated fibroblasts is a hot research field in recent years and alpha-smooth muscular actin(?-SMA)is an important marker of the tumor associated fibroblasts.The extra cellular matrix is an important barrier against the invasion and metastasis of malignant tumor cell.The matrix metalloproteinase-3(MMP-3)has the function to degrade the extra cellular matrix,and then is related to the tumor invasion and metastasis.In recent years,there are a lot of reports about the relationship between the ?-SMA and MMP-3 and the tumor development,invasion and metastasis in domestics and foreign literatures,but there are few studies on breast cancer.To further evaluate the role of ?-SMA and MMP-3 in the development,invasion and metastasis of breast cancer,this study was undertaken to compare the expression of ?-SMA and MMP-3among the tissues of breast cancer,normal breast and breast fibroadenoma and to analyze the relationship between the expression and the biological behavior of breast cancer in clining stage,lymph node metastasis and to analyze between the two proteins.Method: The tissue specimens were collected from in-hospital patients in Affiliated Hosptial of Chengde Medical College andChengde central hospital surgery from May 2012 to September 2014,which included 112 cases of breast cancer tissue specimens,56 cases of normal breast tissue and 69 cases of breast fibroadenoma.All the patients were diagnosed by pathology.The protein expressions of?-SMA and MMP-3 in the specimens were detected by immunohistochemical SP method.The clinical data of the patients were collected from the medical records.Results:1 The expression of ?-SMA and MMP-3 proteins in breast cancer and its correlation with clinical features of breast cancer.The positive expression ?-SMA and MMP-3 proteins in had no significant difference between the normal breast and breast fibroadenoma tissues.The expression of ?-SMA and MMP-3proteins in breast cancer tissues was significantly higher than that in the normal breast and fibroadenoma tissues(P<0.05).In the breast cancer group,the ?-SMA and MMP-3 protein expressions were significantly higher in the clinical stage of III and IV groups than those in the clinical stage of I and II groups(P<0.05),and were significantly higher in the lymph node metastasis group than those in non-lymph node metastasis group(P<0.05).The expressions of?-SMA and MMP-3 proteins were not related to the age of the patient,the diameter of the tumor and the histological grade.2 Correlation between the expression of ?-SMA and MMP-3 in breast cancer tissues.The expression of ?-SMA protein was significantly correlated with that of MMP-3 protein with a correlation coefficient of 0.370(P <0.05).Conclusion:1 The expression of MMP-3 and ?-SMA proteins in breast cancer tissue was significantly higher than that in normal breast and firbroadenoma tissue,suggesting that the two are involved in breastcancer development process.2 The expression of ?-SMA and MMP-3 proteins in breast cancer tissue is closely related to clinical stage,lymph node metastasis,suggesting that both of them play an important role in the invasion and metastasis of breast cancer.3 The expression of the ?-SMA and MMP-3 in the breast cancer tissne was significantly positive correlation,which suggested that theye have a positive synergistic effect in the occurrence and development of breast cancer. |
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