Identification Method Of Chinese Medicine Decoction Piece By Mid-Infrared Spectroscopy

Chinese medicine decoction piece which processed has difference in effectiveness, medicinal properties and toxicity. So should not be confused. At the same time, the character change a lot after processing, it's difficulting to identify. Their infrared spectrum is a variety of chemical composition of the comprehensive reflection, can be used to identify Chinese medicine decoction piece quickly. In this study, the following four respects were researched by combining FTIR with pattern recognition models and statistics:Firstly, Mid-Infrared spectrum finge-rprint to identify armand clematis stem (Chuanmutong).24 samples of Chuanmutong samples and 7 related herbs were collected and tested by Fourier Transform Infrared (FTIR), building the spectrum fingerprint of Chuanmutong, and the limited level (Mean-3?) to identify true or false Chuanmutong by correlation coefficient of 1D-IR was confirmed. Then, Guamnutong and Mutong samples were used as validation samples to evaluate the identification efficacy. The result shows that Chuanmutong can be identified from Mutong and Guamnutong for the established 1R fingerprint. The limited level (0.9926) is exact. It is concluded that the FTIR fingerprint developed can be rapidly and accurately identify Chinese medicine decoction piece.Secondly. Mid-Infrared spectroscopy combined with principal component analysis (PCA) to distinguish different decoction piece of Pinelliae rhizoma.3 Pinelliae rhizome (SBX),12 pinelliae rhizoma praeparatum FBX),11 pinelliae rhizoma praeparatum cum zingibere et alumine (JBX) and 6 pinelliae rhizoma praeparatum cum alumine samples were collected and tested. Their 1D-FTIR were different, SBX located at 1540 cm-1, FBX located on 1616 cm'1, JBX located on 1519 cm-1, QBX located on 1720 cm'1. The spike and correlation coefficient were different of 4 kinds of samples. The PCA shows that clustering different region with no overlap. SD-FTIR of 4 kinds of samples has difference also verfy the variance of 1D-FTIR. It shows that different decoction piece of Pinellia ternata can be identified by Mid-Infrared spectroscopy combined with PCA.Thirdly, Mid-Infrared spectroscopy combined with SIMCA to distinguish aconiti radix and aconiti lateralis radix praeparata. Collected and tested 11 samples of aconiti radix (CW),9 samples of aconiti lateralis radix praeparata (FP).1D-FTIR of 6 CW and 6 FP were selected to build SIMCA clustering analysis method, then the rest of CW and FP samples vertify tne model, the rate of identification and rejection is 100%. The 1D-FTIR and SD-FTIR had their characteristic peaks. It shows that SIMCA of FTIR can be used to identify these 2 kinds of decoction piece.Fourth, Ttri-step IR analyse rhizoma coptidis and wine rhizoma coptidis. Collected and tested 10 rhizoma coptidis (HL) and 7 wine rhizoma coptidis (JHL) by ID, SD,2D-COS FTIR spectroscopy. It's difficult to identify HL and JHL for their 1D-FTIR were exactly similar. The relative intensity of 1508,1387, i363 cm-1?of SD-FTIR was slightly different, so it's still different to identify. The relative intensity of 1499,1570,1609,1650 cm-1 of 2D-COS FTIR were different to identify HL and JHL.