Study Of Preparation Process And Quality Standard Of Qingying Keli

QingYing decoction is from treatise on differentiation and treatment of epidemic febrile disease.With the development of the science of seasonal febrile diseases and perfect,QingYing decoction revered as the science of seasonal ferbrille diseases treatment of hot points of the camp classic,composed of horn(instead of buffalo horn),radix scrophulariae,radix rehmanniae,radix ophiopogonis,honeysuckle,bamboo,rhizome coptidis,forsythia,salvia miltiorrhiza,with the efficacy of clearing the camp breathable,attending evil at camp points,hot and no sleepping at night,having a delirious speech,upset,or macula faint,purple and dry tongue and pulse condition of count.QingYing decoction has obvious effects:antipyretic anti-inflammatory immune regulating antioxidant,a certain degree on blood rheology and myocardial damage.For example,there are obvious therapeutic effect on diabetes vascular lesions,early diabetic kidney disease,thromboangiitis obliterans,burns.At present,it not only used for clinical camp points card,more used in the treatment of gas camp with disease,especially in not sensitive to antibiotics such as western medicine with two burnt gas camp disease,that can obtain the result that expect is less than.For example,an acute contagious infectious diseases,autoimmune disease,skin disease.In the whole process,we made the extraction process and molding process,the quality of the reseatch component of the quality standards,and stability test for finished products,and results show that the preparation process is reasonable,the quality is satble and controllable.1.All the material in the experiment carried out with medicinal fate-based identification,control indicator composition containing the determination of the ingredients,all medicines are in line with the Chinese Pharmacopoeia 2015 edition's relevant requirements.2.Preparation of the research(1)Extraction Process:Through consulting relevant literature,on the basis of the function and treatment as well as the characters of the component of the herbs,and morden pharmaceuticaltechnology,buffalo horn first fried,salvia miltiorrhiza alcohol carry fat-soluble components in advance,flos lonicerae oil extraction,then we draw up water as the extraction solvent.Though single factor test and L9(34)orthogonal test,soak time and amount of water,extraction time,extraction times are imvestigated to determine the best extraction conditions:the final optimization of extraction process of selection,extract three times,add 8 times water,each extraction 1 hours.Experience in certification testing and test amplification,the extraction process is stable and feasible.(2)Molding Process:In accordance with Chinese medicine Preparation of the technical requirements for the forming process of the study,we study the alcohol sink enrichment,granulating,drying temperature in the process of preparation of granules.The results of the alcohol sink enrichment should be controlld at 50%,70?stress concentration(0.07-0.09Mpa),to extract 1;salvia miltiorrhiza alcohol extract recovery of ethanol extract 2,and then mix the two extract.Using concrete:powdered sugar:dextrin:microcrystalline cellulose= 1:3.5:1:0.5granulating,60?drying,mix inclusion compound and volatile oil of flos loicerae whole grains.Preparation of a whole is very smooty,reasonable and stable.3.Product quality standardsWe studied the QingYing particles' s quality standards,according to the Pharmacopoeia general items to check.Including patticle size,moisture,pellen melting,the load,heavy metals,arsenic salts,the results are in line with the Pharmacopoeia.Study on TLC identification:Test used to identify each other in the thin-layer radix scrophulariae,rhizoma coptidis,salvia miltiorrhiza,forsythia,etc.We pretreatment radix rehmanniae,radix scrophulariae,rhizoma coptidis,forsythia,s reference substance,the sample,negative control,and when we put them in the same thin layerboard,using different method coloring,the spots are clear,and no negative interference.Study on content of HPLC determination:Catalpa alcohol was determined by high performance liquid chromatography.The chromatographic conditions are as follows:Eighteen alkyl silane bonded silica as filling agent:Acetonitrile-0.1% phosphoric acid(1:99)as mobile phase;detection wavelength is 210nm;column temperature is 30?;flow rate is 1ml/min.Through the ten batch of pilot study to determine the content of tetrahydropalmatine in the finished product,that is not less than 0.16%.4.Stability testWe detectionde three batches of Qingying Keli,including factors influencing the test and accelerated test.The results show that the product stability is good,and clinical reagent be carried out.