Study The Lipid-lowering Effect Of The Compatibility Of Coptis And Evodia Rutaecarpa Through Leptin/JAK2/STAT3 Signal Pathway

Objective:Set hyperlipidemia as the background, hyperlipemia model rat as the research object, Lipid lowering effect and Leptin/JAK2/STAT3 pathway as target to investigate the lipid-lowering effect of the Compatibility of coptis and Evodia Rutaecarpa.To reveal the mechanism of the Compatibility of coptis and Evodia Rutaecarpa treat hyperlipemia and to explore the scientific connotation of the Compatibility of coptis and Evodia Rutaecarpa.Method:1 Study the Opposite and Complementary Effect of the Compatibility of Coptis and Evodia Rutaecarpa From the Validity of Lipid MetabolismUsed high fat diet to establish hyperlipidemia model of rats. When serum TC was two times of the blank group and the model was successfully established Screening of the successful model, and they were randomly divided into model group, simvastatin group, high and low dose group of BBR, high and low dose group of Evo, high and low dose group of compatibility of BBR and Evo group. The last two group were called high dose group of compatibility and low dose group of compatibility for short. Each group contained 8 rats,contain vacuity group,there were 9 groups. Except the rats in the vacuity group and model group were giving 5%oCMC, other groups were giving the corresponding concentration of drugs by gavage for 60 days. Then serum lipid and fat in the liver were measured. Lipid-lowering effects of the BBR and Evo separate or combined application were compared under the same dose conditions.2 Study the synergism Effect of the Compatibility of Coptis and Evodia Rutaecarpa through the Mechanism of Regulating Lipid MetabolismSelecting healthy male SD rats, method of establishing model, giving drug and sampling methods were the same as first points of the METHOD. Serum Leptin, HMG-CoA reductase and cyp7al in the liver were detected by elisa. P-OB-Rb, p-JAK2 and p-STAT3 in the liver were detected by western blot. OB-Rb, JAK2 and STAT3 mRNA were detected by real time-PCR. The effect of BBR and Evo separate or combined application on Leptin/JAK2/STAT3 signal pathway were compared under the same dose conditions.Result:1 Study the effectiveness of the compatibility of BBR and Evo in regulating the lipid metabolismCompared with the model group, TC and TG level in serum of rats in the medication group was decreased. HDL level was increased. Hepatocyte fatty degeneration was improved. The degree of the liver fibrosis and the formation of pseudo-lobule were reduced. The liver index and liver TG were decreased. And the level of liver TC was decreased except the compatibility groups.Serum TC, degree of liver fibrosis and liver index of the high dose group of compatibility was lower than high dose group of BBR and high dose group of Evo. And liver index of the low dose group of compatibility was lower than low dose group of BBR and low dose group of Evo.2 Research on the mechanism of the compatibility of BBR and Evo in regulating lipid metabolismCompared with the model group, after the last administration serum Leptin level of the rats was increased with statistically significance (P<0.01). Expression of liver p-OB-Rb and OB-Rb mRNA were increased. Expression of liver p-JAK2 and JAK2 mRNA were increased, except the compatibility groups. Expression of liver p-STAT3, STAT3 mRNA and cyp7al were increased. Liver HMG-CoA reductase level was decreased,Liver cyp7a1, p-OB-Rb, p-STAT3,OB-Rb mRNA and STAT3 mRNA levels of high dose group of compatibility were higher than that of BBR high dose group and Evo high dose group.Serum Leptin, liver p-STAT3 and STAT3 mRNA level of low dose group of compatibility was higher than that of BBR low dose group and Evo low dose group, and liver HMG-CoA reductase content was lower than the low dose group of BBR and low dose group of Evo.Conclusion:The compatibility of BBR and Evo showed a synergistic effect in decreasing serum TC, liver index, improve liver fibrosis and most of the target in Leptin/JAK2/STAT3 pathway.The compatibility of Coptis and Evodia Rutaecarpa was opposite and complementary, synergism in coordination in the aspect of regulating lipid and mechanism of lipid metabolism. Acrid openning and bitter descending compatibility of coptis and evodia rutaecarpa has scientific significance.