The Early Diagnosis In Collagen-induced Arthritis Rats By ¹⁸F-NaF Micro-PET/CT

Objective: The changes of bone metabolism in ¹⁸F-NaF micro-PET/CT imaging in collagen-induced arthritis?CIA?model were observed and compared with semi-quantitative arthritis index score,CT score for histopathology score was the gold standard.To evaluate the diagnostic value of 18 NaF micro-PET/CT imaging in the early stages of rheumatoid arthritis,so as to provide scientific experimental data for the early diagnosis of rheumatoid arthritis by ¹⁸F-NaF PET/CT imaging.Methods: 70 healthy female SD rats were randomly selected for normal control group?group A?,50 rats were established CIA model?group B?,The arthritis index was scored at 20 days in the control group and the model group,and the tail vein was injected with 0.1m Ci/kg ¹⁸F-NaF,50 minutes later,the micro-PET/CT imaging was performed,the SUVmin,SUVmax,SUVmean,arthritis index and ?CT score was measured and the severity of rheumatoid arthritis was assessed by histopathology?Three tissue sections were obtained for each ankle joint,and each slice was scored by three pathologists,averaging?.1,arthritis index score,?CT score and ¹⁸F-NaF micro-PET/CT imaging SUV values were compared with the hispathological score for Pearson correlation analysis,P<0.05 indicates that the correlation coefficient is statistically significant,that there is a linear correlation between the two groups;2,the model group?B group?by hispathological score is divided into Group??0 score?,group??0 1 score,including 1 score?,group??1 2 score,including 2 score?,group??2 3 score,including 3 score?.Group ??early histopathology?to remove the arthritis index score or ?CT score>0 points?positive?as C group.The ¹⁸F-NaF micro-PET/CT imaging SUVmin,SUVmax,SUVmean value of group C were compared with those of control group?group A?respectively.Using two independent samples of the t test,P<0.05 for the difference was statistically significant.Results: 1,The correlation analysis the SUVmin,SUVmax,SUVmean of the ¹⁸F-NaF micro-PET/CT imaging,clinical arthritis index score and ?CT score with histopathological score showed that the correlation coefficients were statistically significant?r=0.92,0.94,0.92,0.77,0.77,P<0.001?.And the correlation coefficient r of SUV of ¹⁸F-NaF micro-PET/CT imaging with histopathology score bigger than the clinical arthritis index score and ?CT score.2,The SUVmin,SUVmax and SUVmean values of ¹⁸F-NaF micro-PET/CT imaging in group C were significantly different from those in control group?group A??t=-7.87,-15.97,-9.37,P<0.001?.Conclusion: 1,The ¹⁸F-NaF micro-PET/CT imaging of the SUV value,clinical arthritis index and ?CT can diagnose RA and The correlation between SUV values of ¹⁸F-NaF micro-PET/CT with histopathology was higher than that of clinical arthritis index and ?CT score.2,¹⁸F-NaF micro-PET/CT imaging of the SUV value were positive in the clinical arthritis index score or ?CT score were negative when the early of RA and ¹⁸F-NaF micro-PET/CT diagnosis of early RA is more reliable,intuitive and quantitative analysis of rheumatoid arthritis early changes in bone tissue metabolism.