The human adipose stem cell(hASC)has been highly concerned,since it was found by Zuk PA in 2001.Because of its broader fat tissue sources;easier procedures to obtain and higher yield,when compared to bone marrow derived MSCs,hASC has been widely used in several important biomedical research areas including the stem cell research and regenerative medicine.Given the importance of adipogenesis,the process of stem cells differentiated into adipocytes,for maintaining self-renewal and homeostasis of adipose tissue,coordinating lipid and glucose homeostasis and so on,the mechanistic details of adipogenesis,especially its early adipogenesis stages remains unclear,for example,the early molecular events that promote commitment of mesenchymal precursor cells to the adipogenic lineage.In the present work,we attempt to find a new mechanism of adipogenesis based on the research of regulators during hASC adipogenesis.In recent years,rapid development of Chromatin Immunoprecipitation(ChIP)and high-through genomic sequencing technologies have been transformative for epigenetic and development studies.Our lab has recently developed sensitive tools for genomic deep-seq analysis,which can be used to recognize local enrichment signals of different ChIP-seq data under different signal patterns sensitively and can integrate these local enrichment signals into a compound enrichment signal regions containing biological significances provided for later analysis.In our work,we use epigenetic methods together with this innovative and sensitive algorithm created by our laboratory to study the development of hASC adipogenesis by integrated analysis of ChIP-Seq datasets of H3K4me3,H3K27me3,H3K27ace,H3K36me3 at 3 time points during hASC adipogenesis.Based on the change of chromatin’s activating/repressing situation and transcription factor binding sites,we found profound epigenetic changes on a series of coding or non-coding regions.Finally,we find the gene coding cartilage oligomeric matrix protein(COMP)has an influence on the process of hASC adipogenesis,which reveals a novel role of this protein in regulating hASC adipogenesis.And our study provides new insights into the molecular mechanism of adipogenesis. |