| Women's postmenopausal decline due to ovarian function,estrogen levels plummeted,the body bone turnover rate was significantly faster,bone resuscitation than the rate of bone formation,leading to loss of bone mass,bone mineral density decreased skeletal osteoporosis caused by osteoporosis call postmenopausal osteoporosis(PMOP).Women's postmenopausal estrogen secretion decreased,accelerated bone loss,PMOP has become a serious harm to the public health of high incidence of disease,but also the international bone disease research in the hot and difficult.This study was to investigate the effect of estradiol on bone tissue,uterus and vaginal structure injury in postmenopausal osteoporosis rats and the effect of estradiol on the proliferation of bone tissue mast cells in osteoporotic rats,and to explore the recovery effect of estradiol on bone tissue and reproductive system injury in osteoporosis rats and the possibility of taking mast cells as a basis for the diagnosis and treatment of postmenopausal osteoporosis.In this study,rats with postmenopausal osteoporosis were constructed with three-month-old SD rats and then treated with intraperitoneal injection of estradiol for therapeutic administration.At the end of administration,the rats were executed with cervical dislocation,calculated the organ index.We measured the serum calcium content by flame atomic absorption method,and measured the estradiol(E2)and alkaline phosphatase(ALP)levels in serum by ELISA method.We made paraffin sections after femur,tibia,femoral head tissue fixation and decalcification,and performed H&E staining and toluidine blue staining.The number of bone mast cells in bone tissue was microscopically measured with morphometric analysis in Image-Pro Plus analysis system.H&E staining was performed after taking uterine and vaginal tissue conventional paraffin sections,and we microscopically observed uterine and vaginal histopathological changes,and performed the morphometric analysis.The results showed that serum Ca in the ovariectomized group was significantly lower than that in the sham operation group(P<0.05),E2 significantly decreased(P<0.01),ALP significantly increased(P<0.01).The uterine index of OVX group was significantly lower than that of Sham group(P<0.01).Compared with Sham group,the percentage of femoral metaphyseal and femoral head trabecular area and the thickness of trabecular bone in OVX group significantly decreased(P<0.01),and the trabecular spacing significantly increased(P<0.01).Compared with Sham group,the number of mast cells in femur and femoral head cross-section significantly increased in OVX group(P<0.01).The thickness of uterine diameter of OVX group significantly decreased(P<0.01),the thickness of uterine mucosa and the number of uterine glands significantly decreased(P<0.05).The thickness of vaginal mucosa and the number of muscular vessels significantly decreased in OVX group(P<0.01),and the number of lamina propria vessels significantly decreased(P<0.05).By the results of the study to get the following conclusion: three-month-old SD rats were able to establish a model of postmenopausal osteoporosis disease 3 months after ovariectomy.Intermittent intraperitoneal injection of estradiol can effectively improve the bone metabolism biochemical markers and bone tissue microstructure of ovariectomized rats with osteoporosis,and have the effect of prevention and treatment of postmenopausal osteoporosis.Estradiol can effectively inhibit the proliferation of bone tissue mast cells in ovariectomized rats with osteoporosis.Bone tissue mast cells are expected to be the basis for the diagnosis and treatment of postmenopausal osteoporosis.Estradiol can effectively relieve the uterine and vaginal atrophy of ovariectomized rats with osteoporosis,and have repair effect of reproductive system injury in rats with postmenopausal osteoporosis. |
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