Study On Preparation Process And Quality Standard Of Xuanqi-huayu Concentrated Pills

Objective: To research the preparation technology and establish the quality standards of Xuanqi-huayu concentrated pills.Methods:(1)Using single factors experiment with central comosite design-response surface method,the contents of Astragaloside Ⅳ,Hapagoside,total polysaccharides and extraction rate as index.The extracting time,extraction times and water addition times were investigated for optimizing the extraction process parameters of Astragali radix and other medicinal materials.(2)To research the best molding technology of Xuanqi-huayu concentrated pills.(3)Using thin-layer chromatography identify all Chinese medicinal materials of Xuanqi-huayu concentrated pills.(4)Using high performance liquid chromatography method for determining the content of Astragaloside Ⅳ,Hapagoside and Salvianolic acid B.The methodology was investigate,and the quality standards was established.Result:(1)The optimum extraction conditions of Xuanqi-huayu concentrated pills were 2 times with 13 times water for 130 minutes.(2)The optimum moulding process of Xuanqi-huayu concentrated pills were as follows: Notoginseng radix and Salvia miltiorrhizae radix were crushed into fine power,reserved.The aqueous extract was concentrated to a thick paste with a relative density of 1.35 to 1.40(60 ℃).Take 1/3 thick paste reserve,the rest of the thick paste dryed under reduced pressure at 60 ℃.(3)Established a thin-layer chromatography method for the identification of Xuanqi-huayu concentrated pills in Astragali radix,Notoginseng radix,Scrophulariae radix,Salvia miltiorrhizae radix.(4)Using high performance liquid chromatography method for determining the content of Astragaloside Ⅳ,Hapagoside and Salvianolic acid B.The chromatographic conditions of Astragaloside Ⅳ were as follows: the mobile phase was acetonitrile-water(33:67),evaporative light-scattering detector,the N2 pressure is 40 psl,the drift tube temperature is 60 ℃,the flow rate was 1 m L/min.The chromatographic conditions of Hapagoside and salvianolic B were as follows: the mobile phase was methanol-1 % methanoic acid(45:55),the detection wavelength of UV detector was 278 nm and 286 nm,respectively,the column temperature was 30 ℃,the flow rate was 1 mL/min.Conclusion: The preparation process of Xuanqi-huayu concentrated pills was reasonable and feasible;established quality standard was simple,reliable and stable,can effectively control the quality of the preparation.