| Objective: The purpose of this study is to analyze the differential proteins through proteomic studies comparing the differentially expressed proteins in the development and progression of esophageal cancer.This study is also to investigate the expressions of Wnt2,GSK3? and ?-catenin in NEE(normal esophageal epithelium)and ESCC(Esophageal Squamous Cell carcinoma)in vitro.Methods: Using iTRAQ,the fresh esophageal cancer tissues and normal tissues were labeled respectively,and the biological information and the important signal transduction pathways were analyzed by comparing the two differentially expressed proteins.Cell culture,Wound-healing assay,RT-PCR and Western blot were performed to detect the expressions of Wnt2,?-catenin and GSK3?,and observe the effects on the Wnt/?-catenin pathway.Results: A total of 431 differentially expressed proteins were detected by iTRAQ.The up regulation proteins and down regulated proteins were 72 and 57,respectively.In this study,it was found that the expression of Wnt2,GSK3?,?-catenin in RT-PCR test were consistent with the result in Western blot test.At the same time,the expression of Wnt2 and GSK3? was higher and lower,respectively,in ESCC cell than in NEE cells.However,it was not clear whether the expression of ?-catenin was higher in NEE cells in vitro.Conclusion: a large number of differentially expressed proteins were detected by iTRAQ and provided a reliable resource for biomarkers.Wnt2 and GSK3? were involved in ESCC development through the Wnt/?-catenin pathway.And further research is necessary to explore the ESCC-related mechanism of the WNT/?-catenin signaling pathway. |
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