Protective Effects Of Magnesium Isoglycyrrhizinate On The Injury Induced By Propylthiouracil In Human HepG2 Cells

Objective To study the protective mechanism of propylthiouracil(PTU)on T4 intervention in human Hep G2 cells(simulated in hyperthyroid environment)and to explore the protective effect of magnesium isoglycyrrhizinate(Mg IG)on propylthiouracil-induced injury of human Hep G2 cells at the cellular level.Methods 1,Hep G2 cells were cultured in vitro at a concentration of 10 n M in T4,and incubated with PTU 0,75,150,300,600,1200,2400 ?g / ml for a different time.Hepatocyte damage was induced by hydrogen peroxide(H2O2)as a positive control.MTT colorimetric assay and LDH method were used to detect the cell proliferation and cytotoxicity.The activity of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)were also determined.Finally,we found that the optimal PTU induced Hep G2 cell injury concentration,and established PTU-induced model of hyperthyroid liver injury in vitro.2,Hep G2 cells were divided into control group,positive control group,PTU injury group,Mg IG protection group.The cells in the positive control group were induced with H2O2,and the PTU injury group and the protective group were pretreated with the best damage concentration of PTU.After 8 hours,the original culture medium was discarded and the Mg IG protective group was added into the medium containing 5.0mg / ml Mg IG Incubate for 24 hours,and other groups were supplemented with equal volume of culture medium.AST and ALT were measured by biochemical analyzer.The activities of superoxide dismutase(SOD)and the contents of glutathione(GSH)and malondialdehyde(MDA)were measured by the kit.The protective effect of magnesium isoglycyrrhizinate on liver injury induced by PTU was studied.3,Taking the PTU injury model as the research object,Western blot was used to detect the expression of Bax protein and Bcl-2 protein,the expression of c-Jun N-terminal kinase(JNK),P38 mitogen-activated protein kinase(P38)and its phosphorylated protein.Results 1,PTU on Hep G-2 cells increased with the increase of dose and time.After 8 h of PTU intervention at a concentration of 600 ?g / ml,the inhibition rate of Hep G2 cells was 55.35 ± 0.45% and the mortality rate was 49.16 ± 0.49%.The levels of ALT and AST in culture supernatant were significantly higher than those in normal group(P <0.05).2,Compared with the injury group,cell survival rate was significantly higher after pretreatment with Mg IG(P <0.05).Mg IG could also decrease the intracellular MDA level(P <0.05),and increase the activity of SOD and the content of GSH in Hep G2 cell(P <0.05).3,Western blot analysis showed that PTU could increase the expression of JNK,P38 and phosphorylated protein in Hep G2 cells.Compared with the injury group,the expression of Bax protein and the expression of Bcl-2 protein were significantly increased(P <0.05).Conclusion 1,The optimal concentration and time of establishing PTU-induced Hep G2 cell injury model were 600?g / ml and 8h respectively.2,The mechanism of PTU-induced liver injury may be related to the activation of JNK and p38 MAPK pathway.3,The effect of Mg IG on the damage of Hep G2 cells induced by PTU.